Prevalence of CRISPR-Cas Systems and Their Possible Association with Antibiotic Resistance in Enterococcus faecalis and Enterococcus faecium Collected from Hospital Wastewater

• Main Authors: Alduhaidhawi AHM, AlHuchaimi SN, Al- Mayah TA, Al-Ouqaili MTS, Alkafaas SS, Muthupandian S, Saki M
• Format: Article in Journal/Newspaper
• Language: English
• Published: Dove Medical Press 2022
• Subjects: antibiotic resistance; crispr-cas; enterococcus faecalis; enterococcus faecium; hospital wastewater; hww; Infectious and parasitic diseases; RC109-216; sami
• Online Access: https://doaj.org/article/cf8ca3840d104a039621a490bb81477f

Athraa Harjan Mohsen Alduhaidhawi,1 Sundus Nsaif AlHuchaimi,2 Thikra Abdullah Al- Mayah,2 Mushtak TS Al-Ouqaili,3 Samar Sami Alkafaas,4 Saravanan Muthupandian,5,6 Morteza Saki7 1Department of Biology, Faculty of Science, University of Kufa, Najaf, Iraq; 2Department of Parasitology, Faculty of Medicine, University of Kufa, Najaf, Iraq; 3Department of Microbiology, College of Medicine, University of Anbar, Ramadi, Al-Anbar Governorate, Iraq; 4Department of Chemistry, Division of Biochemistry, Faculty of Science, Tanta University, Tanta, Egypt; 5Department of Microbiology and Immunology, Division of Biomedical Sciences, School of Medicine, College of Health Sciences, Mekelle University, Mekelle, 1871, Ethiopia; 6Department of Pharmacology, AMR and Nanomedicine Laboratory, Center for Transdisciplinary Research, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, 60007, India; 7Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCorrespondence: Saravanan Muthupandian, Department of Microbiology and Immunology, Division of Biomedical Sciences, School of Medicine, College of Health Sciences, Mekelle University, Mekelle, 1871, Ethiopia, Tel [phone], Email [e-mail] Morteza Saki, Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Tel [phone], Email [e-mail]: This study aimed to evaluate the presence of CRISPR-Cas system genes and their possible association with antibiotic resistance patterns of Enterococcus faecalis and Enterococcus faecium species isolated from hospital wastewater (HWW) samples of several hospitals.Methods: HWW samples (200 mL) were collected from wastewater discharged from different hospitals from October 2020 to March 2021. The isolation and identification of enterococci species were performed by standard bacteriology tests and polymerase chain reaction (PCR). Antibiotic resistance was ...