Immunomodulatory effect of ethanol-soluble oligopeptides from Atlantic cod (Gadus morhua)

There are many active substances in Atlantic cod (Gadus morhua) explaining the variety of biological activities. In order to study the immunomodulatory activity and the mechanism of Atlantic cod peptides at the cellular level. In this study, cod peptides were isolated by 80 % ethanol extraction meth...

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Bibliographic Details
Published in:Food Science and Human Wellness
Main Authors: Zhen Yuan, Meilian Yang, Dongyang Zhu, Di Wu, Shuzhen Cheng, Chao Wu, Hesham R. El-Seedi, Ming Du
Format: Article in Journal/Newspaper
Language:English
Published: Tsinghua University Press 2023
Subjects:
Gadus morhua
Immunomodulation
Peptides
TLR2
Molecular docking
Nutrition. Foods and food supply
TX341-641
atlantic cod
Online Access:https://doi.org/10.1016/j.fshw.2022.10.002
https://doaj.org/article/cdf4e6efc8ac4f95abfd84371bdf3870
Description
Summary:There are many active substances in Atlantic cod (Gadus morhua) explaining the variety of biological activities. In order to study the immunomodulatory activity and the mechanism of Atlantic cod peptides at the cellular level. In this study, cod peptides were isolated by 80 % ethanol extraction method, the isolated ethanol-soluble cod peptides (CP-ES) were investigated and their immunomodulatory activity was verified. Additionally, CP-ES showed lower molecular weight and more hydrophobic amino acids. CP-ES could promote the proliferation of spleen lymphocytes and T lymphocytes in mice, suggesting that CP-ES may regulate adaptive immunity. It promoted the release of NO and the expression of iNOS, TNF-α, IL-6 and IL-1β genes in macrophages, suggesting that CP-ES may regulate innate immunity. CP-ES could promote the expression of TLR2 gene, and the peptides identified in CP-ES were docked with TLR2 to predict the peptides playing a major role in CP-ES. These results suggested that CP-ES may regulate the immune activity of both innate and adaptive lines.

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