Use of a combination strategy to improve neuroprotection and neuroregeneration in a rat model of acute spinal cord injury

Spinal cord injury is a very common pathological event that has devastating functional consequences in patients. In recent years, several research groups are trying to find an effective therapy that could be applied in clinical practice. In this study, we analyzed the combination of different strate...

Full description

Bibliographic Details
Published in:Neural Regeneration Research
Main Authors: Elisa García, Roxana Rodríguez-Barrera, Vinnitsa Buzoianu-Anguiano, Adrian Flores-Romero, Emanuel Malagón-Axotla, Marco Guerrero-Godinez, Estefanía De la Cruz-Castillo, Laura Castillo-Carvajal, Monserrat Rivas-Gonzalez, Paola Santiago-Tovar, Ivis Morales, Cesar Borlongan, Antonio Ibarra
Format: Article in Journal/Newspaper
Language:English
Published: Wolters Kluwer Medknow Publications 2019
Subjects:
fibrin glue
mesenchymal stem cells
glial scar
protective autoimmunity
neural derived peptides
neural regeneration
Neurology. Diseases of the nervous system
RC346-429
SCAR
Online Access:https://doi.org/10.4103/1673-5374.250627
https://doaj.org/article/cd678240fa99419a8d3336f7e0ab322a
Description
Summary:Spinal cord injury is a very common pathological event that has devastating functional consequences in patients. In recent years, several research groups are trying to find an effective therapy that could be applied in clinical practice. In this study, we analyzed the combination of different strategies as a potential therapy for spinal cord injury. Immunization with neural derived peptides (INDP), inhibition of glial scar formation (dipyridyl: DPY), as well as the use of biocompatible matrix (fibrin glue: FG) impregnated with bone marrow mesenchymal stem cells (MSCs) were combined and then its beneficial effects were evaluated in the induction of neuroprotection and neuroregeneration after acute SCI. Sprague-Dawley female rats were subjected to a moderate spinal cord injury and then randomly allocated into five groups: 1) phosphate buffered saline; 2) DPY; 3) INDP + DPY; 4) DPY+ FG; 5) INDP + DPY + FG + MSCs. In all rats, intervention was performed 72 hours after spinal cord injury. Locomotor and sensibility recovery was assessed in all rats. At 60 days after treatment, histological examinations of the spinal cord (hematoxylin-eosin and Bielschowsky staining) were performed. Our results showed that the combination therapy (DPY+ INDP + FG + MSCs) was the best strategy to promote motor and sensibility recovery. In addition, significant increases in tissue preservation and axonal density were observed in the combination therapy group. Findings from this study suggest that the combination theapy (DPY+ INDP + FG + MSCs) exhibits potential effects on the protection and regeneration of neural tissue after acute spinal cord injury. All procedures were approved by the Animal Bioethics and Welfare Committee (approval No. 178544; CSNBTBIBAJ 090812960) on August 15, 2016.

Similar Items