Molecular Changes in Circulating microRNAs’ Expression and Oxidative Stress in Adults with Mild Cognitive Impairment: A Biochemical and Molecular Study

• Main Authors: Al-Rawaf HA, Alghadir AH, Gabr SA
• Format: Article in Journal/Newspaper
• Language: English
• Published: Dove Medical Press 2021
• Subjects: circulating mirnas; mci; biomarkers; cellular oxidative stress; apoptosis; real-time rt-pcr; Geriatrics; RC952-954.6; sami
• Online Access: https://doaj.org/article/ccd43b6945b5410c8997837787fb5931

Hadeel A Al-Rawaf,1,2 Ahmad H Alghadir,1 Sami A Gabr1 1Rehabilitation Research Chair, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia; 2Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi ArabiaCorrespondence: Sami A GabrRehabilitation Research Chair (RRC), King Saud University, Riyadh, Kingdom of Saudi ArabiaTel [phone]Fax [phone]Email [e-mail]: The release of miRNAs in tissue fluids significantly recommends its use as non-invasive diagnostic biomarkers for the progression and pathogenesis of mild cognitive impairment (MCI) in aged patients.Objective: The potential role of circulated miRNAs in the pathogenesis of MCI and its association with cellular oxidative stress, apoptosis, and circulated BDNF, Sirtuin 1 (SIRT1), and dipeptidyl peptidase-4 (DPP4) were evaluated in older adults with MCI.Methods: A total of 150 subjects aged 65.4± 3.7 years were recruited in this study. The participants were classified into two groups: healthy normal (n=80) and MCI (n=70). Real-time PCR analysis was performed to estimate the relative expression of miRNAs; miR-124a, miR-483-5p, miR-142-3p, and miR-125b, and apoptotic-related genes Bax, Bcl-2, and caspase-3 in the sera of MCI and control subjects. In addition, oxidative stress parameters; MDA, NO, SOD, and CAT; as well as plasma DPP4 activity, BDNF, SIRT1 levels were colorimetrically estimated.Results: The levels of miR-124a and miR-483-5p significantly increased and miR-142-3p and miR-125b significantly reduced in the serum of MCI patients compared to controls. The expressed miRNAs significantly correlated with severe cognitive decline, measured by MMSE, MoCA, ADL, and memory scores. The expression of Bax, and caspase-3 apoptotic inducing genes significantly increased and Bcl-2 antiapoptotic gene significantly reduced in MCI subjects compared to controls. In addition, the plasma levels of MDA, NO, and DPP4 activity ...