Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success
Abstract Background Bartonella spp. cause persistent bacterial infections in mammals. Although these bacteria are transmitted by blood-feeding arthropods, there is also evidence for vertical transmission in their mammalian hosts. We aimed to determine: (i) the prevalence and diversity of Bartonella...
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ftdoajarticles:oai:doaj.org/article:caa87123144c40acaa4aa8576564870b 2023-05-15T17:12:40+02:00 Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success Katarzyna Tołkacz Mohammed Alsarraf Maciej Kowalec Dorota Dwużnik Maciej Grzybek Jerzy M. Behnke Anna Bajer 2018-08-01T00:00:00Z https://doi.org/10.1186/s13071-018-3047-6 https://doaj.org/article/caa87123144c40acaa4aa8576564870b EN eng BMC http://link.springer.com/article/10.1186/s13071-018-3047-6 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-018-3047-6 1756-3305 https://doaj.org/article/caa87123144c40acaa4aa8576564870b Parasites & Vectors, Vol 11, Iss 1, Pp 1-15 (2018) Babesia microti Bartonella Congenital infection Microtus Poland Vector-borne zoonotic diseases Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s13071-018-3047-6 2022-12-31T00:10:27Z Abstract Background Bartonella spp. cause persistent bacterial infections in mammals. Although these bacteria are transmitted by blood-feeding arthropods, there is also evidence for vertical transmission in their mammalian hosts. We aimed to determine: (i) the prevalence and diversity of Bartonella spp. in a Microtus spp. community; (ii) whether vertical transmission occurs from infected female voles to their offspring; (iii) the effect of concurrent Babesia microti infection on the success of vertical transmission of Bartonella; and (iv) the impact of congenital infection on pup survival. Results We sampled 124 Microtus arvalis, 76 Microtus oeconomus and 17 Microtus agrestis. In total, 115 embryos were isolated from 21 pregnant females. In the following year 11 pregnant females were kept until they had given birth and weaned their pups (n = 62). Blood smears and PCR targeting the Bartonella-specific rpoB gene fragment (333bp) were used for the detection of Bartonella. Bartonella DNA was detected in 66.8% (145/217) of the wild-caught voles. Bartonella infection was detected in 81.8% (36/44) of pregnant female voles. Bartonella-positive individuals were identified among the embryos (47.1%; 40/85) and in 54.8% (34/62) of pups. Congenitally acquired Bartonella infections and co-infection with B. microti had no impact on the survival of pups over a 3-week period post partum. Among 113 Bartonella sequences, four species were detected: Bartonella taylorii, Bartonella grahamii, Bartonella doshiae and a Bartonella rochalimae-like genotype. Bartonella taylorii clade B was the dominant species in wild-caught voles (49%), pregnant females (47%), their embryos (85%), dams (75%) and pups (95%). Conclusions High prevalence of Bartonella spp. infection maintained in Microtus spp. community is followed by a high rate of vertical transmission of several rodent species of Bartonella in three species of naturally infected voles, M. arvalis, M. oeconomus and M. agrestis. Congenitally acquired Bartonella infection does not affect ... Article in Journal/Newspaper Microtus arvalis Directory of Open Access Journals: DOAJ Articles Parasites & Vectors 11 1 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
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English |
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Babesia microti Bartonella Congenital infection Microtus Poland Vector-borne zoonotic diseases Infectious and parasitic diseases RC109-216 |
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Babesia microti Bartonella Congenital infection Microtus Poland Vector-borne zoonotic diseases Infectious and parasitic diseases RC109-216 Katarzyna Tołkacz Mohammed Alsarraf Maciej Kowalec Dorota Dwużnik Maciej Grzybek Jerzy M. Behnke Anna Bajer Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success |
topic_facet |
Babesia microti Bartonella Congenital infection Microtus Poland Vector-borne zoonotic diseases Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Bartonella spp. cause persistent bacterial infections in mammals. Although these bacteria are transmitted by blood-feeding arthropods, there is also evidence for vertical transmission in their mammalian hosts. We aimed to determine: (i) the prevalence and diversity of Bartonella spp. in a Microtus spp. community; (ii) whether vertical transmission occurs from infected female voles to their offspring; (iii) the effect of concurrent Babesia microti infection on the success of vertical transmission of Bartonella; and (iv) the impact of congenital infection on pup survival. Results We sampled 124 Microtus arvalis, 76 Microtus oeconomus and 17 Microtus agrestis. In total, 115 embryos were isolated from 21 pregnant females. In the following year 11 pregnant females were kept until they had given birth and weaned their pups (n = 62). Blood smears and PCR targeting the Bartonella-specific rpoB gene fragment (333bp) were used for the detection of Bartonella. Bartonella DNA was detected in 66.8% (145/217) of the wild-caught voles. Bartonella infection was detected in 81.8% (36/44) of pregnant female voles. Bartonella-positive individuals were identified among the embryos (47.1%; 40/85) and in 54.8% (34/62) of pups. Congenitally acquired Bartonella infections and co-infection with B. microti had no impact on the survival of pups over a 3-week period post partum. Among 113 Bartonella sequences, four species were detected: Bartonella taylorii, Bartonella grahamii, Bartonella doshiae and a Bartonella rochalimae-like genotype. Bartonella taylorii clade B was the dominant species in wild-caught voles (49%), pregnant females (47%), their embryos (85%), dams (75%) and pups (95%). Conclusions High prevalence of Bartonella spp. infection maintained in Microtus spp. community is followed by a high rate of vertical transmission of several rodent species of Bartonella in three species of naturally infected voles, M. arvalis, M. oeconomus and M. agrestis. Congenitally acquired Bartonella infection does not affect ... |
format |
Article in Journal/Newspaper |
author |
Katarzyna Tołkacz Mohammed Alsarraf Maciej Kowalec Dorota Dwużnik Maciej Grzybek Jerzy M. Behnke Anna Bajer |
author_facet |
Katarzyna Tołkacz Mohammed Alsarraf Maciej Kowalec Dorota Dwużnik Maciej Grzybek Jerzy M. Behnke Anna Bajer |
author_sort |
Katarzyna Tołkacz |
title |
Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success |
title_short |
Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success |
title_full |
Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success |
title_fullStr |
Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success |
title_full_unstemmed |
Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success |
title_sort |
bartonella infections in three species of microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent babesia microti infection on its success |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s13071-018-3047-6 https://doaj.org/article/caa87123144c40acaa4aa8576564870b |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Parasites & Vectors, Vol 11, Iss 1, Pp 1-15 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s13071-018-3047-6 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-018-3047-6 1756-3305 https://doaj.org/article/caa87123144c40acaa4aa8576564870b |
op_doi |
https://doi.org/10.1186/s13071-018-3047-6 |
container_title |
Parasites & Vectors |
container_volume |
11 |
container_issue |
1 |
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1766069452948897792 |