Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting

Mapping multiple glycan species to the same peptide and location from shared retention time of glycopeptides with MS and HPLC, Chalk, Greenland et al. construct a detailed mass-retention time database for Spike protein glycopeptides. This allows any mass LC-MS laboratory to reliably identify and qua...

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Bibliographic Details
Published in:Communications Biology
Main Authors: Rod Chalk, William E. P. Greenland, Tiago Moreira, Jesse Coker, Shubhashish M. M. Mukhopadhyay, Eleanor Williams, Charlotte Manning, Tina Bohstedt, Rama McCrorie, Alejandra Fernandez-Cid, Nicola A. Burgess-Brown
Format: Article in Journal/Newspaper
Language:English
Published: Nature Portfolio 2021
Subjects:
Biology (General)
QH301-705.5
Greenland
Online Access:https://doi.org/10.1038/s42003-021-02455-w
https://doaj.org/article/c526a34029cf4619809766a292866602
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Summary:Mapping multiple glycan species to the same peptide and location from shared retention time of glycopeptides with MS and HPLC, Chalk, Greenland et al. construct a detailed mass-retention time database for Spike protein glycopeptides. This allows any mass LC-MS laboratory to reliably identify and quantify Spike protein glycopeptides from a single overnight elastase protein digest in less than 90 minutes.

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