Neuroprotection by misoprostol against rotenone-induced neurotoxicity in rat brain
Objective: To investigate the effect of the prostaglandin E1 analogue misoprostol on oxidative stress and neurodegeration caused by subcutaneous rotenone administration in rats. Methods: Rotenone was administered in a dose of 1.5 mg/kg every other day for 2 weeks. Starting from the 1st day of roteno...
Published in: | Asian Pacific Journal of Tropical Medicine |
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Wolters Kluwer Medknow Publications
2018
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Online Access: | https://doi.org/10.4103/1995-7645.223532 https://doaj.org/article/c2685d7943f749b6a8c1f4219a2919a6 |
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ftdoajarticles:oai:doaj.org/article:c2685d7943f749b6a8c1f4219a2919a6 2023-05-15T15:15:35+02:00 Neuroprotection by misoprostol against rotenone-induced neurotoxicity in rat brain Omar M.E. Abdel-Salam Amany A Sleem Eman R Youness Nadia A Mohammed Enayat A Omara 2018-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.223532 https://doaj.org/article/c2685d7943f749b6a8c1f4219a2919a6 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=1;spage=40;epage=47;aulast=Abdel-Salam https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.223532 https://doaj.org/article/c2685d7943f749b6a8c1f4219a2919a6 Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 1, Pp 40-47 (2018) misoprostol rotenone brain oxidative stress b cell/lymphoma-2 paraoxonase Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.4103/1995-7645.223532 2022-12-31T02:54:30Z Objective: To investigate the effect of the prostaglandin E1 analogue misoprostol on oxidative stress and neurodegeration caused by subcutaneous rotenone administration in rats. Methods: Rotenone was administered in a dose of 1.5 mg/kg every other day for 2 weeks. Starting from the 1st day of rotenone injection, rats were subcutaneously treated with misoprostol at doses of 10, 100 or 1 000 μ g/kg. Rats were evaluated for brain lipid peroxidation (malondialdehyde: MDA), reduced glutathione (GSH), nitric oxide (NO) levels, and paraoxonase-1 (PON-1) activity. The concentrations of the anti-apoptotic protein B cell/lymphoma-2 (Bcl-2) were determined in the striatum. Histopathologic examination and the expression of inducible nitric oxide synthase (iNOS) in the cerebral cortex and striatum were also performed. Results: Compared with the vehicle-treated group, rotenone caused a significant increase in brain lipid proxidation (MDA) by 61% (P<0.05) accompanied by an increase in NO by 73.1% (P<0.05) and a decrease in GSH concentration by 29.4% (P<0.05). In addition, brain PON-1 activity significantly decreased by 63.0% (P<0.05) and striatal Bcl-2 significantly decreased by 27.9% (P<0.05) with respect to the corresponding control value. Brain sections from rotenone treated rats showed extensive dark pyknotic and apoptotic nuclei in neurons, shrunken cytoplasm and perineuronal vacuolation. Rotenone also caused pronounced expression of iNOS in the cerebral cortex and striatum. Treatment with misoprostol at doses of 100 and 1 000 μ g/kg resulted in decreased brain MDA (by 16.5%-23.0%) (P<0.05) and NO levels (by 37.1%-40.7%) (P<0.05) and increased GSH concentrations (by 18.8%-30.1%) (P<0.05). PON-1 activity was significantly increased by 80.0%-114.8% (P<0.05) by misoprostol at 100 and 1 000 μ g/kg, respectively. In addition, misoprostol treatment restored striatal Bcl-2 concentrations to its normal value. Misoprostol treatment resulted in markedly reduced brain injury and decreased iNOS expression ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 11 1 40 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
misoprostol rotenone brain oxidative stress b cell/lymphoma-2 paraoxonase Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
misoprostol rotenone brain oxidative stress b cell/lymphoma-2 paraoxonase Arctic medicine. Tropical medicine RC955-962 Omar M.E. Abdel-Salam Amany A Sleem Eman R Youness Nadia A Mohammed Enayat A Omara Neuroprotection by misoprostol against rotenone-induced neurotoxicity in rat brain |
topic_facet |
misoprostol rotenone brain oxidative stress b cell/lymphoma-2 paraoxonase Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To investigate the effect of the prostaglandin E1 analogue misoprostol on oxidative stress and neurodegeration caused by subcutaneous rotenone administration in rats. Methods: Rotenone was administered in a dose of 1.5 mg/kg every other day for 2 weeks. Starting from the 1st day of rotenone injection, rats were subcutaneously treated with misoprostol at doses of 10, 100 or 1 000 μ g/kg. Rats were evaluated for brain lipid peroxidation (malondialdehyde: MDA), reduced glutathione (GSH), nitric oxide (NO) levels, and paraoxonase-1 (PON-1) activity. The concentrations of the anti-apoptotic protein B cell/lymphoma-2 (Bcl-2) were determined in the striatum. Histopathologic examination and the expression of inducible nitric oxide synthase (iNOS) in the cerebral cortex and striatum were also performed. Results: Compared with the vehicle-treated group, rotenone caused a significant increase in brain lipid proxidation (MDA) by 61% (P<0.05) accompanied by an increase in NO by 73.1% (P<0.05) and a decrease in GSH concentration by 29.4% (P<0.05). In addition, brain PON-1 activity significantly decreased by 63.0% (P<0.05) and striatal Bcl-2 significantly decreased by 27.9% (P<0.05) with respect to the corresponding control value. Brain sections from rotenone treated rats showed extensive dark pyknotic and apoptotic nuclei in neurons, shrunken cytoplasm and perineuronal vacuolation. Rotenone also caused pronounced expression of iNOS in the cerebral cortex and striatum. Treatment with misoprostol at doses of 100 and 1 000 μ g/kg resulted in decreased brain MDA (by 16.5%-23.0%) (P<0.05) and NO levels (by 37.1%-40.7%) (P<0.05) and increased GSH concentrations (by 18.8%-30.1%) (P<0.05). PON-1 activity was significantly increased by 80.0%-114.8% (P<0.05) by misoprostol at 100 and 1 000 μ g/kg, respectively. In addition, misoprostol treatment restored striatal Bcl-2 concentrations to its normal value. Misoprostol treatment resulted in markedly reduced brain injury and decreased iNOS expression ... |
format |
Article in Journal/Newspaper |
author |
Omar M.E. Abdel-Salam Amany A Sleem Eman R Youness Nadia A Mohammed Enayat A Omara |
author_facet |
Omar M.E. Abdel-Salam Amany A Sleem Eman R Youness Nadia A Mohammed Enayat A Omara |
author_sort |
Omar M.E. Abdel-Salam |
title |
Neuroprotection by misoprostol against rotenone-induced neurotoxicity in rat brain |
title_short |
Neuroprotection by misoprostol against rotenone-induced neurotoxicity in rat brain |
title_full |
Neuroprotection by misoprostol against rotenone-induced neurotoxicity in rat brain |
title_fullStr |
Neuroprotection by misoprostol against rotenone-induced neurotoxicity in rat brain |
title_full_unstemmed |
Neuroprotection by misoprostol against rotenone-induced neurotoxicity in rat brain |
title_sort |
neuroprotection by misoprostol against rotenone-induced neurotoxicity in rat brain |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/1995-7645.223532 https://doaj.org/article/c2685d7943f749b6a8c1f4219a2919a6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 1, Pp 40-47 (2018) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=1;spage=40;epage=47;aulast=Abdel-Salam https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.223532 https://doaj.org/article/c2685d7943f749b6a8c1f4219a2919a6 |
op_doi |
https://doi.org/10.4103/1995-7645.223532 |
container_title |
Asian Pacific Journal of Tropical Medicine |
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11 |
container_issue |
1 |
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40 |
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1766345954420588544 |