Comparative study of cultivation of feces in vermiculite or charcoal to obtain larvae of Strongyloides venezuelensis

Introduction We compared feces culturing in charcoal or vermiculite to obtain Strongyloides venezuelensis larvae. Methods Feces (5g) from infected rats was mixed with vermiculite (10g) or coal (10g) in plastic cups and incubated at 28°C for 48h. Larvae were recovered using Baermann-Moraes method. Re...

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Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical
Main Authors: Steveen Rios Ribeiro, Caroline Ohnesorge Maia, Fausto Edmundo Lima Pereira, Narcisa Imaculada Brant Moreira
Format: Article in Journal/Newspaper
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2014
Subjects:
Charcoal
Strongyloides
Vermiculite
Arctic medicine. Tropical medicine
RC955-962
Arctic
Online Access:https://doi.org/10.1590/0037-8682-0019-2014
https://doaj.org/article/c1419bcaebed4571aa8469e0c53451c9
Description
Summary:Introduction We compared feces culturing in charcoal or vermiculite to obtain Strongyloides venezuelensis larvae. Methods Feces (5g) from infected rats was mixed with vermiculite (10g) or coal (10g) in plastic cups and incubated at 28°C for 48h. Larvae were recovered using Baermann-Moraes method. Results Significantly higher number of positive larval cultures were recovered from vermiculite than from charcoal (15/17 and 4/17, respectively; p < 0.001; 990.6 ± 307.5 and 215 ± 78.1 larvae, p = 0.027). Conclusions Vermiculite yields more larvae and provides cleaner pellets, improving larvae identification and facilitating their use for other purposes.

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