Extraction, Identification, Modification, and Antibacterial Activity of Histone from Immature Testis of Atlantic salmon

In the present study, histone from immature testis of Atlantic salmon was extracted and identified, and its antibacterial activity after enzymolysis was investigated. Histone extracted from Atlantic salmon ( Salmo salar ) testis using the acid extraction method was successfully identified by LC-MS/M...

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Bibliographic Details
Published in:Marine Drugs
Main Authors: Boyu Fu, Hong Lin, Tushar Ramesh Pavase, Nasha Mi, Jianxin Sui
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2020
Subjects:
atlantic salmon ( salmo salar )
histone
extraction
antibacterial activity
enzymatic hydrolysis
Biology (General)
QH301-705.5
Atlantic salmon
Salmo salar
Online Access:https://doi.org/10.3390/md18030133
https://doaj.org/article/c0f5de293dfc4165ad6f47128a428d66
Description
Summary:In the present study, histone from immature testis of Atlantic salmon was extracted and identified, and its antibacterial activity after enzymolysis was investigated. Histone extracted from Atlantic salmon ( Salmo salar ) testis using the acid extraction method was successfully identified by LC-MS/MS, and revealed significant inhibitory activity on both the Gram-negative and Gram-positive bacteria. With a low concentration of 10 mg/mL, the observed inhibitory zone diameter (IZD) could significantly reach up to 15.23 mm. After modification of enzymatic hydrolysis by pepsin, histone could be digested to three fragments, while the antibacterial activity increased up to 57.7%. All the results suggested the leftovers from commercial fishing could be utilized for the extraction of antimicrobial peptides.

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