Overestimating resistance in field testing of malaria parasites: simple methods for estimating high EC 50 values using a Bayesian approach
Abstract Conventional methods of assessing in-vitro antimalarial drug-concentration effect relationships in field testing of fresh isolates assess each parasite isolate individually. This leads to systematic overestimation of EC 50 values for the most resistant isolates, and thus overestimation of t...
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ftdoajarticles:oai:doaj.org/article:b6df19e3749e43599f986904ae02102d 2023-05-15T15:01:38+02:00 Overestimating resistance in field testing of malaria parasites: simple methods for estimating high EC 50 values using a Bayesian approach Brockman Alan Chotivanich Kesinee Stepniewska Kasia Day Nicholas PJ White Nicholas J 2007-01-01T00:00:00Z https://doi.org/10.1186/1475-2875-6-4 https://doaj.org/article/b6df19e3749e43599f986904ae02102d EN eng BMC http://www.malariajournal.com/content/6/1/4 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-6-4 1475-2875 https://doaj.org/article/b6df19e3749e43599f986904ae02102d Malaria Journal, Vol 6, Iss 1, p 4 (2007) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2007 ftdoajarticles https://doi.org/10.1186/1475-2875-6-4 2022-12-30T22:25:28Z Abstract Conventional methods of assessing in-vitro antimalarial drug-concentration effect relationships in field testing of fresh isolates assess each parasite isolate individually. This leads to systematic overestimation of EC 50 values for the most resistant isolates, and thus overestimation of the degree of resistance. In antimalarial drug-susceptibility studies conducted on the north-western border of Thailand the overestimation of EC 50 for the most resistant isolate ranged from 15% for artesunate to 43% for mefloquine. If isolates cannot be stored for re-testing, more accurate estimations of the degree of resistance can be obtained using a Bayesian approach to data analysis which is described here. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 6 1 4 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Brockman Alan Chotivanich Kesinee Stepniewska Kasia Day Nicholas PJ White Nicholas J Overestimating resistance in field testing of malaria parasites: simple methods for estimating high EC 50 values using a Bayesian approach |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Conventional methods of assessing in-vitro antimalarial drug-concentration effect relationships in field testing of fresh isolates assess each parasite isolate individually. This leads to systematic overestimation of EC 50 values for the most resistant isolates, and thus overestimation of the degree of resistance. In antimalarial drug-susceptibility studies conducted on the north-western border of Thailand the overestimation of EC 50 for the most resistant isolate ranged from 15% for artesunate to 43% for mefloquine. If isolates cannot be stored for re-testing, more accurate estimations of the degree of resistance can be obtained using a Bayesian approach to data analysis which is described here. |
format |
Article in Journal/Newspaper |
author |
Brockman Alan Chotivanich Kesinee Stepniewska Kasia Day Nicholas PJ White Nicholas J |
author_facet |
Brockman Alan Chotivanich Kesinee Stepniewska Kasia Day Nicholas PJ White Nicholas J |
author_sort |
Brockman Alan |
title |
Overestimating resistance in field testing of malaria parasites: simple methods for estimating high EC 50 values using a Bayesian approach |
title_short |
Overestimating resistance in field testing of malaria parasites: simple methods for estimating high EC 50 values using a Bayesian approach |
title_full |
Overestimating resistance in field testing of malaria parasites: simple methods for estimating high EC 50 values using a Bayesian approach |
title_fullStr |
Overestimating resistance in field testing of malaria parasites: simple methods for estimating high EC 50 values using a Bayesian approach |
title_full_unstemmed |
Overestimating resistance in field testing of malaria parasites: simple methods for estimating high EC 50 values using a Bayesian approach |
title_sort |
overestimating resistance in field testing of malaria parasites: simple methods for estimating high ec 50 values using a bayesian approach |
publisher |
BMC |
publishDate |
2007 |
url |
https://doi.org/10.1186/1475-2875-6-4 https://doaj.org/article/b6df19e3749e43599f986904ae02102d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 6, Iss 1, p 4 (2007) |
op_relation |
http://www.malariajournal.com/content/6/1/4 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-6-4 1475-2875 https://doaj.org/article/b6df19e3749e43599f986904ae02102d |
op_doi |
https://doi.org/10.1186/1475-2875-6-4 |
container_title |
Malaria Journal |
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6 |
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1 |
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4 |
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1766333638573555712 |