Overestimating resistance in field testing of malaria parasites: simple methods for estimating high EC 50 values using a Bayesian approach

Abstract Conventional methods of assessing in-vitro antimalarial drug-concentration effect relationships in field testing of fresh isolates assess each parasite isolate individually. This leads to systematic overestimation of EC 50 values for the most resistant isolates, and thus overestimation of t...

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Bibliographic Details
Published in:Malaria Journal
Main Authors: Brockman Alan, Chotivanich Kesinee, Stepniewska Kasia, Day Nicholas PJ, White Nicholas J
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2007
Subjects:
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1186/1475-2875-6-4
https://doaj.org/article/b6df19e3749e43599f986904ae02102d
Description
Summary:Abstract Conventional methods of assessing in-vitro antimalarial drug-concentration effect relationships in field testing of fresh isolates assess each parasite isolate individually. This leads to systematic overestimation of EC 50 values for the most resistant isolates, and thus overestimation of the degree of resistance. In antimalarial drug-susceptibility studies conducted on the north-western border of Thailand the overestimation of EC 50 for the most resistant isolate ranged from 15% for artesunate to 43% for mefloquine. If isolates cannot be stored for re-testing, more accurate estimations of the degree of resistance can be obtained using a Bayesian approach to data analysis which is described here.

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