Extended-spectrum β-lactamase-producing Salmonella enterica serovar Oranienburg (CTX-M-2 group) in a pediatric hospital in Tucumán, Argentina Salmonella enterica serovar Oranienburg produtora de β-lactamase de espectro extendido (grupo CTX-M-2) em um hospital pediátrico de Tucumán, Argentina

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: María Angela Jure, Olga Aulet, Ana Trejo, Marta Castillo
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2010
• Subjects: Salmonella enterica serovariedade Oranienburg; β-lactamasas de espectro extendido; Salmonella enterica serovar Oranienburg; Extended Spectrum β-lactamases; Arctic medicine. Tropical medicine; RC955-962; Arctic; Argentina; Tucumán
• Online Access: https://doi.org/10.1590/S0037-86822010000200003; https://doaj.org/article/b4406da000fe40f685608c8285146805

INTRODUCTION: Salmonella sp infections have been reported over recent years in hospitals in Argentina and other countries due to multiresistant strains. The aim of this study was to characterize the extended-spectrum β-lactamases in third-generation cephalosporin-resistant strains of Salmonella enterica serovar Oranienburg. METHODS: We studied 60 strains isolated from children with gastroenteritis and/or extraintestinal complications. The antibiotic susceptibility patterns of the isolates were analyzed and the β-lactamases were characterized using phenotyping and genotyping methods. RESULTS: All the strains were resistant to ampicillin, cefotaxime, cefepime and aztreonam and partially susceptible to ceftazidime, thus corresponding well with the resistance phenotype conferred by CTX-M-type β-lactamases. An isoelectric point enzyme (pI = 7.9) was detected in all of the strains, and this was confirmed by PCR as a member of the CTX-M-2 group. CONCLUSIONS: This is the first report of Salmonella enterica serovar Oranienburg producing β-lactamases of the CTX-M-2 group in a pediatric hospital in Tucumán, Argentina. INTRODUÇÃO: Em recentes anos foram informadas infecções por Salmonella sp em hospitais da Argentina e outros países devido as cepas multiresistentes. O objetivo deste estudo era caracterizar as β-lactamasas de espectro extendido em cepas de Salmonella enterica serovar Oranienburg resistentes às cefalosporinas de terceira geração. MÉTODOS: Nós estudamos 60 cepas obtidas de pacientes com gastroenterites e com complicações extraintestinais. Os padrões de susceptibilidade antibiotica foram estudados em os isolamentos, as β-lactamasas foram caracterizadas por métodos fenotípicos e genotípicos. RESULTADOS: Todas as cepas eram resistentes a ampicillin, cefotaxime, cefepime e aztreonam e parcialmente suscetível a ceftazidime que corresponde bem com o fenótipo de resistência conferido por as β-lactamasas tipo CTX-M. Na totalidade das cepas, se detectou uma enzima de ponto isoelétrico (pI) 7,9, confirmada por PCR como ...