An efficient method in breaking of dormancy from B unium persicum (Boiss) Fedtsch seeds: a valuable herb of Middle East and Central Asia

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Yousef Emamipoor, Mahmood Maziah
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2014
• Subjects: Bunium persicum; Seed germination; Thidiazuron; Benzyl aminopurine; Gibberellic acid 3; Forchlorfenuron; Chilling temperature; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0042; https://doaj.org/article/b39fac4975b349b89838fd0064ac4ee0

Objective: To develop a protocol for breaking of seed dormancy and increasing the seed germination rate of Bunium persicum. Methods: The seeds were treated with 3.1, 6.3, 12.5, 25, 50 and 100 μmol/L of benzyl aminopurine, gibberellic acid (GA3), thidiazuron (TDZ) and forchlorfenuron. Then, seeds were transferred to two different temperature conditions including room temperature (25 °C) and chilling temperature (2-5 °C). Results: The treatment of moist seeds with chilling temperature (2-5 °C) broke seed dormancy and showed maximum germination, which was 54.7% after 60 d treatment. Also, the treatment of dry seeds with chilling temperature broke seed dormancy with 9.3% germination rate after 120 d. Treatment of seeds with different level of plant growth regulators showed that under moist-room condition, there was evidence of higher and lower seed germination rate: GA3 (100 μmol/L) with 46.7% and TDZ (50 μmol/L) with 6.67% respectively. In addition, the results showed that under moist-chilling condition, TDZ (6.3 μmol/L) with 53.3% seed germination rate had higher influence on breaking seed dormancy. Treatment of seeds with combination of TDZ and GA3 under moist-chilling condition revealed higher rate of breaking of seed dormancy when 6.3 μmol/L TDZ was combined with 100 μmol/L GA3, showing 93.7% germination rate. Conclusions: The effect of plant growth regulators coupled with chilling temperature on breaking of seed dormancy could provide a large number of seedlings while the long juvenile time which is the next restricting factor of plantation still remained. Thus, the subsequent growth of seedlings to provide a large number of corms is necessary for successful plantation.