Piscirickettsia salmonis- Triggered Extracellular Traps Formation as an Innate Immune Response of Atlantic Salmon-Derived Polymorphonuclear Neutrophils
Extracellular traps (ETs) are webs of DNA, citrullinated histones, anti-microbial peptides, and proteins that were not previously reported in Atlantic salmon ( Salmo salar ). ETs are mainly released from polymorphonuclear neutrophils (PMN) and are considered a novel PMN-derived effector mechanism ag...
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ftdoajarticles:oai:doaj.org/article:aeae04da91844e2cbd9385ac95ae29f7 2023-10-01T03:54:34+02:00 Piscirickettsia salmonis- Triggered Extracellular Traps Formation as an Innate Immune Response of Atlantic Salmon-Derived Polymorphonuclear Neutrophils Pablo Alarcon Gabriel Espinosa Catalina Millan Julia Saravia Vania Quinteros Ricardo Enriquez Claudio Henriquez Luis Vargas-Chacoff Rafael A. Burgos Anja Taubert Carlos Hermosilla Francisco J. Morera 2021-03-01T00:00:00Z https://doi.org/10.3390/biology10030206 https://doaj.org/article/aeae04da91844e2cbd9385ac95ae29f7 EN eng MDPI AG https://www.mdpi.com/2079-7737/10/3/206 https://doaj.org/toc/2079-7737 doi:10.3390/biology10030206 2079-7737 https://doaj.org/article/aeae04da91844e2cbd9385ac95ae29f7 Biology, Vol 10, Iss 206, p 206 (2021) extracellular traps Atlantic salmon Piscirickettsia salmonis innate immunity Salmo salar Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/biology10030206 2023-09-03T00:42:36Z Extracellular traps (ETs) are webs of DNA, citrullinated histones, anti-microbial peptides, and proteins that were not previously reported in Atlantic salmon ( Salmo salar ). ETs are mainly released from polymorphonuclear neutrophils (PMN) and are considered a novel PMN-derived effector mechanism against different invasive pathogens. Here, we showed that Atlantic salmon-derived PMN released ETs-like structures in vitro in response to highly pathogenic facultative intracellular rickettsial bacteria Piscirickettsia salmonis . PMN were isolated from pre-smolt Atlantic salmon and stimulated in vitro with oleic acid and P. salmonis. Extracellular DNA was measured using the PicoGreen™ dye, while immunofluorescence image analysis was used to confirm the classical components of salmonid-extruded ETs. Future studies are required to better understand the role of Atlantic salmon-derived ETs orchestrating innate/adaptive immunity and the knowledge on regulation pathways involved in this cell death process. Thus, comprehension of salmonid-derived ETs against P. salmonis might represent novel alternative strategies to improve host innate defense mechanisms of farmed salmon against closely related rickettsial bacteria, as a complement to disease prevention and control strategies. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Biology 10 3 206 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
extracellular traps Atlantic salmon Piscirickettsia salmonis innate immunity Salmo salar Biology (General) QH301-705.5 |
spellingShingle |
extracellular traps Atlantic salmon Piscirickettsia salmonis innate immunity Salmo salar Biology (General) QH301-705.5 Pablo Alarcon Gabriel Espinosa Catalina Millan Julia Saravia Vania Quinteros Ricardo Enriquez Claudio Henriquez Luis Vargas-Chacoff Rafael A. Burgos Anja Taubert Carlos Hermosilla Francisco J. Morera Piscirickettsia salmonis- Triggered Extracellular Traps Formation as an Innate Immune Response of Atlantic Salmon-Derived Polymorphonuclear Neutrophils |
topic_facet |
extracellular traps Atlantic salmon Piscirickettsia salmonis innate immunity Salmo salar Biology (General) QH301-705.5 |
description |
Extracellular traps (ETs) are webs of DNA, citrullinated histones, anti-microbial peptides, and proteins that were not previously reported in Atlantic salmon ( Salmo salar ). ETs are mainly released from polymorphonuclear neutrophils (PMN) and are considered a novel PMN-derived effector mechanism against different invasive pathogens. Here, we showed that Atlantic salmon-derived PMN released ETs-like structures in vitro in response to highly pathogenic facultative intracellular rickettsial bacteria Piscirickettsia salmonis . PMN were isolated from pre-smolt Atlantic salmon and stimulated in vitro with oleic acid and P. salmonis. Extracellular DNA was measured using the PicoGreen™ dye, while immunofluorescence image analysis was used to confirm the classical components of salmonid-extruded ETs. Future studies are required to better understand the role of Atlantic salmon-derived ETs orchestrating innate/adaptive immunity and the knowledge on regulation pathways involved in this cell death process. Thus, comprehension of salmonid-derived ETs against P. salmonis might represent novel alternative strategies to improve host innate defense mechanisms of farmed salmon against closely related rickettsial bacteria, as a complement to disease prevention and control strategies. |
format |
Article in Journal/Newspaper |
author |
Pablo Alarcon Gabriel Espinosa Catalina Millan Julia Saravia Vania Quinteros Ricardo Enriquez Claudio Henriquez Luis Vargas-Chacoff Rafael A. Burgos Anja Taubert Carlos Hermosilla Francisco J. Morera |
author_facet |
Pablo Alarcon Gabriel Espinosa Catalina Millan Julia Saravia Vania Quinteros Ricardo Enriquez Claudio Henriquez Luis Vargas-Chacoff Rafael A. Burgos Anja Taubert Carlos Hermosilla Francisco J. Morera |
author_sort |
Pablo Alarcon |
title |
Piscirickettsia salmonis- Triggered Extracellular Traps Formation as an Innate Immune Response of Atlantic Salmon-Derived Polymorphonuclear Neutrophils |
title_short |
Piscirickettsia salmonis- Triggered Extracellular Traps Formation as an Innate Immune Response of Atlantic Salmon-Derived Polymorphonuclear Neutrophils |
title_full |
Piscirickettsia salmonis- Triggered Extracellular Traps Formation as an Innate Immune Response of Atlantic Salmon-Derived Polymorphonuclear Neutrophils |
title_fullStr |
Piscirickettsia salmonis- Triggered Extracellular Traps Formation as an Innate Immune Response of Atlantic Salmon-Derived Polymorphonuclear Neutrophils |
title_full_unstemmed |
Piscirickettsia salmonis- Triggered Extracellular Traps Formation as an Innate Immune Response of Atlantic Salmon-Derived Polymorphonuclear Neutrophils |
title_sort |
piscirickettsia salmonis- triggered extracellular traps formation as an innate immune response of atlantic salmon-derived polymorphonuclear neutrophils |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/biology10030206 https://doaj.org/article/aeae04da91844e2cbd9385ac95ae29f7 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Biology, Vol 10, Iss 206, p 206 (2021) |
op_relation |
https://www.mdpi.com/2079-7737/10/3/206 https://doaj.org/toc/2079-7737 doi:10.3390/biology10030206 2079-7737 https://doaj.org/article/aeae04da91844e2cbd9385ac95ae29f7 |
op_doi |
https://doi.org/10.3390/biology10030206 |
container_title |
Biology |
container_volume |
10 |
container_issue |
3 |
container_start_page |
206 |
_version_ |
1778522358582083584 |