Atlantic Salmon Mucins Inhibit LuxS -Dependent A. Salmonicida AI-2 Quorum Sensing in an N -Acetylneuraminic Acid-Dependent Manner

One of the most important bacterial diseases in salmonid aquaculture is furunculosis, caused by Aeromonas salmonicida . Bacterial communication through secreted autoinducer signals, quorum sensing, takes part in the regulation of gene expression in bacteria, influencing growth and virulence. The ski...

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Bibliographic Details
Published in:International Journal of Molecular Sciences
Main Authors: János Tamás Padra, Stefany Ojaimi Loibman, Kaisa Thorell, Henrik Sundh, Kristina Sundell, Sara K. Lindén
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2022
Subjects:
sialic acid
signaling
bacterial growth
biofilm
mucus
Biology (General)
QH301-705.5
Chemistry
QD1-999
Atlantic salmon
Online Access:https://doi.org/10.3390/ijms23084326
https://doaj.org/article/ad7e9935ce0046e3b3abf07508867421
Description
Summary:One of the most important bacterial diseases in salmonid aquaculture is furunculosis, caused by Aeromonas salmonicida . Bacterial communication through secreted autoinducer signals, quorum sensing, takes part in the regulation of gene expression in bacteria, influencing growth and virulence. The skin and mucosal surfaces, covered by a mucus layer, are the first point of contact between fish and bacteria. Mucins are highly glycosylated and are the main components of mucus. Here, we validate the Vibrio harveyi BB170 bioreporter assay for quantifying A. salmonicida quorum sensing and study the effects of Atlantic salmon mucins as well as mono- and disaccharides on the AI-2 levels of A. salmonicida . Atlantic salmon mucins from skin, pyloric ceca, proximal and distal intestine reduced A. salmonicida AI-2 levels. Among the saccharides abundant on mucins, fucose, N -acetylneuraminic acid and GlcNAcβ1-3Gal inhibited AI-2 A. salmonicida secretion. Removal of N -acetylneuraminic acid, which is the most abundant terminal residue on mucin glycans on Atlantic salmon mucins, attenuated the inhibitory effects on AI-2 levels of A. salmonicida. Deletion of A. salmonicida luxS abolished AI-2 production. In conclusion, Atlantic salmon mucins regulate A. salmonicida quorum sensing in a luxS and N -acetylneuraminic acid-dependent manner.

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