A versatile nuclei extraction protocol for single nucleus sequencing in non-model species-Optimization in various Atlantic salmon tissues.

The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present...

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Bibliographic Details
Published in:PLOS ONE
Main Authors: Rose Ruiz Daniels, Richard S Taylor, Ross Dobie, Sarah Salisbury, James J Furniss, Emily Clark, Daniel J Macqueen, Diego Robledo
Format: Article in Journal/Newspaper
Language:English
Published: Public Library of Science (PLoS) 2023
Subjects:
Medicine
R
Science
Q
Atlantic salmon
Online Access:https://doi.org/10.1371/journal.pone.0285020
https://doaj.org/article/ab19d3068c284b06a2fe44636f13ed42
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spelling ftdoajarticles:oai:doaj.org/article:ab19d3068c284b06a2fe44636f13ed42 2023-10-09T21:49:56+02:00 A versatile nuclei extraction protocol for single nucleus sequencing in non-model species-Optimization in various Atlantic salmon tissues. Rose Ruiz Daniels Richard S Taylor Ross Dobie Sarah Salisbury James J Furniss Emily Clark Daniel J Macqueen Diego Robledo 2023-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0285020 https://doaj.org/article/ab19d3068c284b06a2fe44636f13ed42 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0285020 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0285020 https://doaj.org/article/ab19d3068c284b06a2fe44636f13ed42 PLoS ONE, Vol 18, Iss 9, p e0285020 (2023) Medicine R Science Q article 2023 ftdoajarticles https://doi.org/10.1371/journal.pone.0285020 2023-09-17T00:37:58Z The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie. This protocol can be successfully applied to extract nuclei from a variety of tissues and species. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles PLOS ONE 18 9 e0285020
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Rose Ruiz Daniels
Richard S Taylor
Ross Dobie
Sarah Salisbury
James J Furniss
Emily Clark
Daniel J Macqueen
Diego Robledo
A versatile nuclei extraction protocol for single nucleus sequencing in non-model species-Optimization in various Atlantic salmon tissues.
topic_facet Medicine
R
Science
Q
description The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie. This protocol can be successfully applied to extract nuclei from a variety of tissues and species.
format Article in Journal/Newspaper
author Rose Ruiz Daniels
Richard S Taylor
Ross Dobie
Sarah Salisbury
James J Furniss
Emily Clark
Daniel J Macqueen
Diego Robledo
author_facet Rose Ruiz Daniels
Richard S Taylor
Ross Dobie
Sarah Salisbury
James J Furniss
Emily Clark
Daniel J Macqueen
Diego Robledo
author_sort Rose Ruiz Daniels
title A versatile nuclei extraction protocol for single nucleus sequencing in non-model species-Optimization in various Atlantic salmon tissues.
title_short A versatile nuclei extraction protocol for single nucleus sequencing in non-model species-Optimization in various Atlantic salmon tissues.
title_full A versatile nuclei extraction protocol for single nucleus sequencing in non-model species-Optimization in various Atlantic salmon tissues.
title_fullStr A versatile nuclei extraction protocol for single nucleus sequencing in non-model species-Optimization in various Atlantic salmon tissues.
title_full_unstemmed A versatile nuclei extraction protocol for single nucleus sequencing in non-model species-Optimization in various Atlantic salmon tissues.
title_sort versatile nuclei extraction protocol for single nucleus sequencing in non-model species-optimization in various atlantic salmon tissues.
publisher Public Library of Science (PLoS)
publishDate 2023
url https://doi.org/10.1371/journal.pone.0285020
https://doaj.org/article/ab19d3068c284b06a2fe44636f13ed42
genre Atlantic salmon
genre_facet Atlantic salmon
op_source PLoS ONE, Vol 18, Iss 9, p e0285020 (2023)
op_relation https://doi.org/10.1371/journal.pone.0285020
https://doaj.org/toc/1932-6203
1932-6203
doi:10.1371/journal.pone.0285020
https://doaj.org/article/ab19d3068c284b06a2fe44636f13ed42
op_doi https://doi.org/10.1371/journal.pone.0285020
container_title PLOS ONE
container_volume 18
container_issue 9
container_start_page e0285020
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