A versatile nuclei extraction protocol for single nucleus sequencing in non-model species-Optimization in various Atlantic salmon tissues.

The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present...

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Bibliographic Details
Published in:PLOS ONE
Main Authors: Rose Ruiz Daniels, Richard S Taylor, Ross Dobie, Sarah Salisbury, James J Furniss, Emily Clark, Daniel J Macqueen, Diego Robledo
Format: Article in Journal/Newspaper
Language:English
Published: Public Library of Science (PLoS) 2023
Subjects:
Medicine
R
Science
Q
Atlantic salmon
Online Access:https://doi.org/10.1371/journal.pone.0285020
https://doaj.org/article/ab19d3068c284b06a2fe44636f13ed42
Description
Summary:The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie. This protocol can be successfully applied to extract nuclei from a variety of tissues and species.

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