Comparison of electrochemiluminescence and ELISA methods in the detection of blood borne pathogens in Gabon

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Cyrille Bisseye, Jophrette Mireille Ntsame Ndong, Anicet Mouity Matoumba, Calixte Bengone, Guy Mouelet Migolet, Bolni Marius Nagalo
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2017
• Subjects: Cobas 6000 e601; EVOLIS BioRad; Kappa coefficient; Blood donors; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/j.apjtb.2017.08.008; https://doaj.org/article/a9ac23b1152c43a9a8df5e7b5e7658e0

Objective: To assess the performances of Cobas 6000 e601 and EVOLIS BioRad in the detection of HIV, HBV and HCV in blood donors in Libreville (Gabon). Methods: A cross-sectional investigation was conducted in July 2017 in a total of 2000 blood donors recruited at the National Blood transfusion Center, Libreville Gabon. Among them, 363 donors were selected to compare the performances of COBAS 6000 e601 (electro-chemiluminescence) and EVOLIS BioRad in detecting HIV, HBV and HCV using Cohen's kappa coefficient. Results: Both methods yielded similar results for the detection of HIV and HBsAg. A very good agreement of 93.39% and an excellent agreement of 98.90% were obtained for the detection of HIV and HbsAg, with kappa values of 0.80 and 0.98, respectively. The observed agreement of 91.86% was found for the detection of HCV, which gave a fair agreement between the two methods with kappa = 0.33. Conclusions: The two evaluation methods showed a similar performance in the detection of HIV, HBV. However, given the high rate of intra and inter-genotypes recombination known for HIV and HBV, more robust techniques of detection such as polymerase chain reaction should be used to prevent post-transfusion contaminations.