Comparison of electrochemiluminescence and ELISA methods in the detection of blood borne pathogens in Gabon
Objective: To assess the performances of Cobas 6000 e601 and EVOLIS BioRad in the detection of HIV, HBV and HCV in blood donors in Libreville (Gabon). Methods: A cross-sectional investigation was conducted in July 2017 in a total of 2000 blood donors recruited at the National Blood transfusion Cente...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2017
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Subjects: | |
Online Access: | https://doi.org/10.1016/j.apjtb.2017.08.008 https://doaj.org/article/a9ac23b1152c43a9a8df5e7b5e7658e0 |
Summary: | Objective: To assess the performances of Cobas 6000 e601 and EVOLIS BioRad in the detection of HIV, HBV and HCV in blood donors in Libreville (Gabon). Methods: A cross-sectional investigation was conducted in July 2017 in a total of 2000 blood donors recruited at the National Blood transfusion Center, Libreville Gabon. Among them, 363 donors were selected to compare the performances of COBAS 6000 e601 (electro-chemiluminescence) and EVOLIS BioRad in detecting HIV, HBV and HCV using Cohen's kappa coefficient. Results: Both methods yielded similar results for the detection of HIV and HBsAg. A very good agreement of 93.39% and an excellent agreement of 98.90% were obtained for the detection of HIV and HbsAg, with kappa values of 0.80 and 0.98, respectively. The observed agreement of 91.86% was found for the detection of HCV, which gave a fair agreement between the two methods with kappa = 0.33. Conclusions: The two evaluation methods showed a similar performance in the detection of HIV, HBV. However, given the high rate of intra and inter-genotypes recombination known for HIV and HBV, more robust techniques of detection such as polymerase chain reaction should be used to prevent post-transfusion contaminations. |
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