A New APEH Cluster with Antioxidant Functions in the Antarctic Hemoglobinless Icefish Chionodraco hamatus.
Acylpeptide hydrolase (APEH) is a ubiquitous cytosolic protease that plays an important role in the detoxification of oxidised proteins. In this work, to further explore the physiological role of this enzyme, two apeh cDNAs were isolated from the Chionodraco hamatus icefish, which lives in the highl...
Published in: | PLOS ONE |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pone.0125594 https://doaj.org/article/a4ce8022459e4dd0a4583d6160c49b12 |
id |
ftdoajarticles:oai:doaj.org/article:a4ce8022459e4dd0a4583d6160c49b12 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:a4ce8022459e4dd0a4583d6160c49b12 2023-05-15T13:50:26+02:00 A New APEH Cluster with Antioxidant Functions in the Antarctic Hemoglobinless Icefish Chionodraco hamatus. Alessia Riccio Marta Gogliettino Gianna Palmieri Marco Balestrieri Angelo Facchiano Mosè Rossi Stefania Palumbo Giuseppe Monti Ennio Cocca 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0125594 https://doaj.org/article/a4ce8022459e4dd0a4583d6160c49b12 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4422685?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0125594 https://doaj.org/article/a4ce8022459e4dd0a4583d6160c49b12 PLoS ONE, Vol 10, Iss 5, p e0125594 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0125594 2022-12-31T08:28:46Z Acylpeptide hydrolase (APEH) is a ubiquitous cytosolic protease that plays an important role in the detoxification of oxidised proteins. In this work, to further explore the physiological role of this enzyme, two apeh cDNAs were isolated from the Chionodraco hamatus icefish, which lives in the highly oxygenated Antarctic marine environment. The encoded proteins (APEH-1(Ch) and APEH-2(Ch)) were characterised in comparison with the uniquely expressed isoform from the temperate fish Dicentrarchus labrax (APEH-1Dl) and the two APEHs from the red-blooded Antarctic fish Trematomus bernacchii (APEH-1(Tb) and APEH-2(Tb)). Homology modelling and kinetic characterisation of the APEH isoforms provided new insights into their structure/function properties. APEH-2 isoforms were the only ones capable of hydrolysing oxidised proteins, with APEH-2(Ch) being more efficient than APEH-2(Tb) at this specific function. Therefore, this ability of APEH-2 isoforms is the result of an evolutionary adaptation due to the pressure of a richly oxygenated environment. The lack of expression of APEH-2 in the tissues of the temperate fish used as the controls further supported this hypothesis. In addition, analysis of gene expression showed a significant discrepancy between the levels of transcripts and those of proteins, especially for apeh-2 genes, which suggests the presence of post-transcriptional regulation mechanisms, triggered by cold-induced oxidative stress, that produce high basal levels of APEH-2 mRNA as a reserve that is ready to use in case of the accumulation of oxidised proteins. Article in Journal/Newspaper Antarc* Antarctic Icefish Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic PLOS ONE 10 5 e0125594 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Alessia Riccio Marta Gogliettino Gianna Palmieri Marco Balestrieri Angelo Facchiano Mosè Rossi Stefania Palumbo Giuseppe Monti Ennio Cocca A New APEH Cluster with Antioxidant Functions in the Antarctic Hemoglobinless Icefish Chionodraco hamatus. |
topic_facet |
Medicine R Science Q |
description |
Acylpeptide hydrolase (APEH) is a ubiquitous cytosolic protease that plays an important role in the detoxification of oxidised proteins. In this work, to further explore the physiological role of this enzyme, two apeh cDNAs were isolated from the Chionodraco hamatus icefish, which lives in the highly oxygenated Antarctic marine environment. The encoded proteins (APEH-1(Ch) and APEH-2(Ch)) were characterised in comparison with the uniquely expressed isoform from the temperate fish Dicentrarchus labrax (APEH-1Dl) and the two APEHs from the red-blooded Antarctic fish Trematomus bernacchii (APEH-1(Tb) and APEH-2(Tb)). Homology modelling and kinetic characterisation of the APEH isoforms provided new insights into their structure/function properties. APEH-2 isoforms were the only ones capable of hydrolysing oxidised proteins, with APEH-2(Ch) being more efficient than APEH-2(Tb) at this specific function. Therefore, this ability of APEH-2 isoforms is the result of an evolutionary adaptation due to the pressure of a richly oxygenated environment. The lack of expression of APEH-2 in the tissues of the temperate fish used as the controls further supported this hypothesis. In addition, analysis of gene expression showed a significant discrepancy between the levels of transcripts and those of proteins, especially for apeh-2 genes, which suggests the presence of post-transcriptional regulation mechanisms, triggered by cold-induced oxidative stress, that produce high basal levels of APEH-2 mRNA as a reserve that is ready to use in case of the accumulation of oxidised proteins. |
format |
Article in Journal/Newspaper |
author |
Alessia Riccio Marta Gogliettino Gianna Palmieri Marco Balestrieri Angelo Facchiano Mosè Rossi Stefania Palumbo Giuseppe Monti Ennio Cocca |
author_facet |
Alessia Riccio Marta Gogliettino Gianna Palmieri Marco Balestrieri Angelo Facchiano Mosè Rossi Stefania Palumbo Giuseppe Monti Ennio Cocca |
author_sort |
Alessia Riccio |
title |
A New APEH Cluster with Antioxidant Functions in the Antarctic Hemoglobinless Icefish Chionodraco hamatus. |
title_short |
A New APEH Cluster with Antioxidant Functions in the Antarctic Hemoglobinless Icefish Chionodraco hamatus. |
title_full |
A New APEH Cluster with Antioxidant Functions in the Antarctic Hemoglobinless Icefish Chionodraco hamatus. |
title_fullStr |
A New APEH Cluster with Antioxidant Functions in the Antarctic Hemoglobinless Icefish Chionodraco hamatus. |
title_full_unstemmed |
A New APEH Cluster with Antioxidant Functions in the Antarctic Hemoglobinless Icefish Chionodraco hamatus. |
title_sort |
new apeh cluster with antioxidant functions in the antarctic hemoglobinless icefish chionodraco hamatus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0125594 https://doaj.org/article/a4ce8022459e4dd0a4583d6160c49b12 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Icefish |
genre_facet |
Antarc* Antarctic Icefish |
op_source |
PLoS ONE, Vol 10, Iss 5, p e0125594 (2015) |
op_relation |
http://europepmc.org/articles/PMC4422685?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0125594 https://doaj.org/article/a4ce8022459e4dd0a4583d6160c49b12 |
op_doi |
https://doi.org/10.1371/journal.pone.0125594 |
container_title |
PLOS ONE |
container_volume |
10 |
container_issue |
5 |
container_start_page |
e0125594 |
_version_ |
1766253488463937536 |