Chromatographic and mass spectrometric analysis of secondary metabolites of Deschampsia antarctica from Galindez Island, Argentine Islands

• Main Authors: Roman Ivannikov, Viktor Anishchenko, Pavlo Kuzema, Oksana Stavinskaya, Iryna Laguta, Oksana Poronnik, Ivan Parnikoza
• Format: Article in Journal/Newspaper
• Language: English
• Published: Polish Academy of Sciences 2022
• Subjects: west antarctic; antarctic hairgrass; polyphenolic antioxidants; flavonoids; luteolin derivatives; Geology; QE1-996.5; Antarctic; Argentine; Argentine Islands; Galindez; Galindez Island; Antarc*; Antarctica; Polar Research
• Online Access: https://doi.org/10.24425/ppr.2022.140369; https://doaj.org/article/a3e8d8f607684c7a9c3cbcb264f9d81e

The aim of this work was to study the polyphenolic composition of Deschampsia antarctica È. Desv. plants grown at natural conditions on different locations on the Galindez Island, Argentine Islands, the maritime Antarctic. The plants were collected during the summer season of the 26th Ukrainian Antarctic Expedition (2020–2022). The extracts of 21 plants were obtained and the composition of the extracts was analyzed by means of high-performance liquid chromatography and matrix-assisted laser desorption/ionization mass spectrometry. The antioxidant properties of the extracts were characterized using the DPPH (2,2-diphenyl-1-picrylhydrazyl) test. The extracts were found to contain large amount of polyphenolic compounds, with flavonoids and phenolic acids, as well as their derivatives, being the most common classes of the phenols. Using the HPLC data the content of various phenols in the plants was systematic studied. It has been found that in all plants the most abundant phenols are flavonoids/flavonoid derivatives (on average about 75% of total mass of phenols). Among the flavonoids, luteolin derivatives predominate (86–94% of the total mass of flavonoids), and, among luteolin derivatives, the main compounds are orientin, orientin 2"- O-β-arabinopyranoside and isoswertiajaponin 2"- O-β-arabinopyranoside (67–83% of the total mass of luteolin derivatives). It has been also found that all the extracts possess the high activity in inhibition of DPPH radicals and that the antioxidant activity of the extracts correlates with total content of phenols in the samples. Thus, Deschampsia antarctica É. Desv. plants are a valuable source of natural phenolic antioxidants, and the most common antioxidants in the extracts are orientin, orientin 2"- O-β-arabinopyranoside and isoswertiajaponin 2"- O-β-arabinopyranoside.