Real-time polymerase chain reaction method for rapid detection of Pangasius bocourti-derived components in fish products
ObjectiveTo establish a real-time polymerase chain reaction (PCR) rapid detection method for Pangasius bocourti-derived components.MethodsPrimers were designed according to the mitochondrial cytb gene sequence of Pangasius bocourti, and real-time PCR was used for amplification to achieve the purpose...
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The Editorial Office of Chinese Journal of Food Hygiene
2021
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ftdoajarticles:oai:doaj.org/article:a1f401976e03438c8e65125809c0ecf1 2023-05-15T15:27:28+02:00 Real-time polymerase chain reaction method for rapid detection of Pangasius bocourti-derived components in fish products LI Jie QIAN Yunkai ZHANG Xin WANG Jiafang LI Xuehong Lin Peng WANG Jianchang SUN Yunda 2021-07-01T00:00:00Z https://doi.org/10.13590/j.cjfh.2021.04.007 https://doaj.org/article/a1f401976e03438c8e65125809c0ecf1 ZH chi The Editorial Office of Chinese Journal of Food Hygiene http://www.zgspws.com/zgspwszz/article/abstract/20210407?st=article_issue https://doaj.org/toc/1004-8456 1004-8456 doi:10.13590/j.cjfh.2021.04.007 https://doaj.org/article/a1f401976e03438c8e65125809c0ecf1 Zhongguo shipin weisheng zazhi, Vol 33, Iss 04, Pp 426-429 (2021) pangasius bocourti real-time polymerase chain reaction fish products adulteration detection Food processing and manufacture TP368-456 Nutrition. Foods and food supply TX341-641 article 2021 ftdoajarticles https://doi.org/10.13590/j.cjfh.2021.04.007 2022-12-30T19:26:53Z ObjectiveTo establish a real-time polymerase chain reaction (PCR) rapid detection method for Pangasius bocourti-derived components.MethodsPrimers were designed according to the mitochondrial cytb gene sequence of Pangasius bocourti, and real-time PCR was used for amplification to achieve the purpose of rapid detection of products.ResultsThis method had good specificity, and the sensitivity could reach 10-4 ng Pangasius bocourti DNA. Pangasius bocourti cytb gene could be detected in fish products mixed with rice noodles, sesame powder, chicken powder and atlantic cod meal, and the sensitivity could reach 0.01%.ConclusionThis method had high specificity, short time and high sensitivity, and could meet the detection requirements of Pangasius bocourti adulteration in fish meat products. Article in Journal/Newspaper atlantic cod Directory of Open Access Journals: DOAJ Articles |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
Chinese |
topic |
pangasius bocourti real-time polymerase chain reaction fish products adulteration detection Food processing and manufacture TP368-456 Nutrition. Foods and food supply TX341-641 |
spellingShingle |
pangasius bocourti real-time polymerase chain reaction fish products adulteration detection Food processing and manufacture TP368-456 Nutrition. Foods and food supply TX341-641 LI Jie QIAN Yunkai ZHANG Xin WANG Jiafang LI Xuehong Lin Peng WANG Jianchang SUN Yunda Real-time polymerase chain reaction method for rapid detection of Pangasius bocourti-derived components in fish products |
topic_facet |
pangasius bocourti real-time polymerase chain reaction fish products adulteration detection Food processing and manufacture TP368-456 Nutrition. Foods and food supply TX341-641 |
description |
ObjectiveTo establish a real-time polymerase chain reaction (PCR) rapid detection method for Pangasius bocourti-derived components.MethodsPrimers were designed according to the mitochondrial cytb gene sequence of Pangasius bocourti, and real-time PCR was used for amplification to achieve the purpose of rapid detection of products.ResultsThis method had good specificity, and the sensitivity could reach 10-4 ng Pangasius bocourti DNA. Pangasius bocourti cytb gene could be detected in fish products mixed with rice noodles, sesame powder, chicken powder and atlantic cod meal, and the sensitivity could reach 0.01%.ConclusionThis method had high specificity, short time and high sensitivity, and could meet the detection requirements of Pangasius bocourti adulteration in fish meat products. |
format |
Article in Journal/Newspaper |
author |
LI Jie QIAN Yunkai ZHANG Xin WANG Jiafang LI Xuehong Lin Peng WANG Jianchang SUN Yunda |
author_facet |
LI Jie QIAN Yunkai ZHANG Xin WANG Jiafang LI Xuehong Lin Peng WANG Jianchang SUN Yunda |
author_sort |
LI Jie |
title |
Real-time polymerase chain reaction method for rapid detection of Pangasius bocourti-derived components in fish products |
title_short |
Real-time polymerase chain reaction method for rapid detection of Pangasius bocourti-derived components in fish products |
title_full |
Real-time polymerase chain reaction method for rapid detection of Pangasius bocourti-derived components in fish products |
title_fullStr |
Real-time polymerase chain reaction method for rapid detection of Pangasius bocourti-derived components in fish products |
title_full_unstemmed |
Real-time polymerase chain reaction method for rapid detection of Pangasius bocourti-derived components in fish products |
title_sort |
real-time polymerase chain reaction method for rapid detection of pangasius bocourti-derived components in fish products |
publisher |
The Editorial Office of Chinese Journal of Food Hygiene |
publishDate |
2021 |
url |
https://doi.org/10.13590/j.cjfh.2021.04.007 https://doaj.org/article/a1f401976e03438c8e65125809c0ecf1 |
genre |
atlantic cod |
genre_facet |
atlantic cod |
op_source |
Zhongguo shipin weisheng zazhi, Vol 33, Iss 04, Pp 426-429 (2021) |
op_relation |
http://www.zgspws.com/zgspwszz/article/abstract/20210407?st=article_issue https://doaj.org/toc/1004-8456 1004-8456 doi:10.13590/j.cjfh.2021.04.007 https://doaj.org/article/a1f401976e03438c8e65125809c0ecf1 |
op_doi |
https://doi.org/10.13590/j.cjfh.2021.04.007 |
_version_ |
1766357895001145344 |