Real-time polymerase chain reaction method for rapid detection of Pangasius bocourti-derived components in fish products

ObjectiveTo establish a real-time polymerase chain reaction (PCR) rapid detection method for Pangasius bocourti-derived components.MethodsPrimers were designed according to the mitochondrial cytb gene sequence of Pangasius bocourti, and real-time PCR was used for amplification to achieve the purpose...

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Bibliographic Details
Main Authors: LI Jie, QIAN Yunkai, ZHANG Xin, WANG Jiafang, LI Xuehong, Lin Peng, WANG Jianchang, SUN Yunda
Format: Article in Journal/Newspaper
Language:Chinese
Published: The Editorial Office of Chinese Journal of Food Hygiene 2021
Subjects:
pangasius bocourti
real-time polymerase chain reaction
fish products
adulteration
detection
Food processing and manufacture
TP368-456
Nutrition. Foods and food supply
TX341-641
atlantic cod
Online Access:https://doi.org/10.13590/j.cjfh.2021.04.007
https://doaj.org/article/a1f401976e03438c8e65125809c0ecf1
Description
Summary:ObjectiveTo establish a real-time polymerase chain reaction (PCR) rapid detection method for Pangasius bocourti-derived components.MethodsPrimers were designed according to the mitochondrial cytb gene sequence of Pangasius bocourti, and real-time PCR was used for amplification to achieve the purpose of rapid detection of products.ResultsThis method had good specificity, and the sensitivity could reach 10-4 ng Pangasius bocourti DNA. Pangasius bocourti cytb gene could be detected in fish products mixed with rice noodles, sesame powder, chicken powder and atlantic cod meal, and the sensitivity could reach 0.01%.ConclusionThis method had high specificity, short time and high sensitivity, and could meet the detection requirements of Pangasius bocourti adulteration in fish meat products.

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