A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding

We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patien...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz
Main Authors: Manoel Barral-Netto, A. Barral
Format: Article in Journal/Newspaper
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 1986
Subjects:
concanavalin
mononuclear cells
visceral leishmaniasis
Arctic medicine. Tropical medicine
RC955-962
Microbiology
QR1-502
Arctic
Online Access:https://doi.org/10.1590/S0074-02761986000300012
https://doaj.org/article/a0037a2dc7a54e10986d617cb82c5f2f
Description
Summary:We report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patients with American leishmaniasis do not interfere with binding of ConA to PBMC.

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