Optimized Enzymatic Synthesis of Hesperidin Fatty Acid Esters in a Two-Phase System Containing Ionic Liquid

Response surface methodology (RSM) based on a five-level, three-variable central composite design (CCD) was employed for modeling and optimizing the conversion yield of the enzymatic acylation of hesperidin with decanoic acid using immobilized Candida antarctica lipase B (CALB) in a two-phase system...

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Bibliographic Details
Published in:Molecules
Main Authors: Yollanda Edwirges Moreira Franco, Alexandra C.H. Frankland Sawaya, Thiago Grando Alberto, Patrícia de Oliveira Carvalho, Natália Dalfré, Fabiano Jares Contesini, Maria Elisa Melo Branco de Araújo
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2011
Subjects:
Candida antarctica
lipase
hesperidin
acylation
Organic chemistry
QD241-441
Antarc*
Antarctica
Online Access:https://doi.org/10.3390/molecules16087171
https://doaj.org/article/9d7222f369f34856a865d1f6472c5f1c
Description
Summary:Response surface methodology (RSM) based on a five-level, three-variable central composite design (CCD) was employed for modeling and optimizing the conversion yield of the enzymatic acylation of hesperidin with decanoic acid using immobilized Candida antarctica lipase B (CALB) in a two-phase system containing [bmim]BF4. The three variables studied (molar ratio of hesperidin to decanoic acid, [bmim]BF4/acetone ratio and lipase concentration) significantly affected the conversion yield of acylated hesperidin derivative. Verification experiments confirmed the validity of the predicted model. The lipase showed higher conversion degree in a two-phase system using [bmim]BF4 and acetone compared to that in pure acetone. Under the optimal reaction conditions carried out in a single-step biocatalytic process when the water content was kept lower than 200 ppm, the maximum acylation yield was 53.6%.

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