Lipase-produced omega-3 acylglycerols for the fortification and stabilization of extra virgin olive oil using hydroxytyrosyl palmitate
Pure concentrates of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were used to produce monoacylglycerol and diacylglycerol (MDG) oils via enzymatic glycerolysis in a solvent-free system. Immobilized lipase from Candida antarctica B (Novozym 435) was employed for the glycerolysis reaction w...
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2021
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ftdoajarticles:oai:doaj.org/article:9cb1665e44ab48d9bf9568cf19b8cf41 2023-05-15T14:04:00+02:00 Lipase-produced omega-3 acylglycerols for the fortification and stabilization of extra virgin olive oil using hydroxytyrosyl palmitate Tharuka Gunathilake Taiwo O. Akanbi Colin J. Barrow 2021-12-01T00:00:00Z https://doi.org/10.1016/j.fufo.2021.100045 https://doaj.org/article/9cb1665e44ab48d9bf9568cf19b8cf41 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2666833521000356 https://doaj.org/toc/2666-8335 2666-8335 doi:10.1016/j.fufo.2021.100045 https://doaj.org/article/9cb1665e44ab48d9bf9568cf19b8cf41 Future Foods, Vol 4, Iss , Pp 100045- (2021) Monoacylglycerol Diacylglycerol Omega-3 Fatty acids Lipase Glycerolysis Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 article 2021 ftdoajarticles https://doi.org/10.1016/j.fufo.2021.100045 2022-12-31T16:32:56Z Pure concentrates of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were used to produce monoacylglycerol and diacylglycerol (MDG) oils via enzymatic glycerolysis in a solvent-free system. Immobilized lipase from Candida antarctica B (Novozym 435) was employed for the glycerolysis reaction with up to 90% product formation in one hour. This lipase also favoured diacylglycerol (DAG) over monoacylglycerol (MAG) production. Products were purified using silica gel chromatography and characterised by capillary chromatography with flame ionization detection (Iatroscan-FID). Purified products were added to extra virgin olive oil (EVOO) at a concentration of 20% w/w. The fortified olive oil was stabilized using hydroxytyrosyl palmitate, an antioxidant prepared by conjugating hydroxytyrosol with palmitic acid using Novozym 435 lipase. In vitro hydrolysis of the fortified oil was also carried out using porcine pancreatic lipase to investigate the mechanism of action of this enzyme on the oil mixtures. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Fid ENVELOPE(-65.939,-65.939,-68.664,-68.664) Future Foods 4 100045 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Monoacylglycerol Diacylglycerol Omega-3 Fatty acids Lipase Glycerolysis Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 |
spellingShingle |
Monoacylglycerol Diacylglycerol Omega-3 Fatty acids Lipase Glycerolysis Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 Tharuka Gunathilake Taiwo O. Akanbi Colin J. Barrow Lipase-produced omega-3 acylglycerols for the fortification and stabilization of extra virgin olive oil using hydroxytyrosyl palmitate |
topic_facet |
Monoacylglycerol Diacylglycerol Omega-3 Fatty acids Lipase Glycerolysis Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 |
description |
Pure concentrates of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were used to produce monoacylglycerol and diacylglycerol (MDG) oils via enzymatic glycerolysis in a solvent-free system. Immobilized lipase from Candida antarctica B (Novozym 435) was employed for the glycerolysis reaction with up to 90% product formation in one hour. This lipase also favoured diacylglycerol (DAG) over monoacylglycerol (MAG) production. Products were purified using silica gel chromatography and characterised by capillary chromatography with flame ionization detection (Iatroscan-FID). Purified products were added to extra virgin olive oil (EVOO) at a concentration of 20% w/w. The fortified olive oil was stabilized using hydroxytyrosyl palmitate, an antioxidant prepared by conjugating hydroxytyrosol with palmitic acid using Novozym 435 lipase. In vitro hydrolysis of the fortified oil was also carried out using porcine pancreatic lipase to investigate the mechanism of action of this enzyme on the oil mixtures. |
format |
Article in Journal/Newspaper |
author |
Tharuka Gunathilake Taiwo O. Akanbi Colin J. Barrow |
author_facet |
Tharuka Gunathilake Taiwo O. Akanbi Colin J. Barrow |
author_sort |
Tharuka Gunathilake |
title |
Lipase-produced omega-3 acylglycerols for the fortification and stabilization of extra virgin olive oil using hydroxytyrosyl palmitate |
title_short |
Lipase-produced omega-3 acylglycerols for the fortification and stabilization of extra virgin olive oil using hydroxytyrosyl palmitate |
title_full |
Lipase-produced omega-3 acylglycerols for the fortification and stabilization of extra virgin olive oil using hydroxytyrosyl palmitate |
title_fullStr |
Lipase-produced omega-3 acylglycerols for the fortification and stabilization of extra virgin olive oil using hydroxytyrosyl palmitate |
title_full_unstemmed |
Lipase-produced omega-3 acylglycerols for the fortification and stabilization of extra virgin olive oil using hydroxytyrosyl palmitate |
title_sort |
lipase-produced omega-3 acylglycerols for the fortification and stabilization of extra virgin olive oil using hydroxytyrosyl palmitate |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://doi.org/10.1016/j.fufo.2021.100045 https://doaj.org/article/9cb1665e44ab48d9bf9568cf19b8cf41 |
long_lat |
ENVELOPE(-65.939,-65.939,-68.664,-68.664) |
geographic |
Fid |
geographic_facet |
Fid |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Future Foods, Vol 4, Iss , Pp 100045- (2021) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2666833521000356 https://doaj.org/toc/2666-8335 2666-8335 doi:10.1016/j.fufo.2021.100045 https://doaj.org/article/9cb1665e44ab48d9bf9568cf19b8cf41 |
op_doi |
https://doi.org/10.1016/j.fufo.2021.100045 |
container_title |
Future Foods |
container_volume |
4 |
container_start_page |
100045 |
_version_ |
1766274938196459520 |