A simple and inexprensive method to generate a microaerophilic atmosphere for the isolation of Campylobacter sp Un método simple y económico para generar la atmósfera microaerofílica para el aislamiento de Campylobacter sp
Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x X in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag,...
| Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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| Main Author: | |
| Format: | Article in Journal/Newspaper |
| Language: | English |
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Universidade de São Paulo (USP)
1996
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| Subjects: | |
| Online Access: | https://doi.org/10.1590/S0036-46651996000300015 https://doaj.org/article/98f290826e664aeda397e632e9d92cc0 |
| Summary: | Faeces of 138 chickens were inoculated on Blaser agar plates. One set of plates was incubated in jars with CampyPak envelopes. The others were incubated in "Zip-lock" plastic bags (7 x X in.) and a microaerophilic atmosphere was generated exhaling into the "Zip-lock" plastic bag, after holding the breath for 20 sec. Then, the bag was pressed to evacuate its atmosphere, inflated again, and pressed (4 times), and finally sealed. Campylobacter was isolated from 127 (96.2%) of samples incubated in jars with gas generator envelopes and from 129 (98%) of the specimens incubated into the bags. The proposed methodology offers good savings for cost-conscious laboratories. Se inocularon por duplicado 138 muestras de heces de pollos en agar de Blaser. Un juego de las placas se incubó en jarras con sobres generadores de microaerobiosis (CampyPak). Las otras placas se incubaron en bolsas plásticas tipo "Zip-lock" (7 x 8 pulgadas) y la atmósfera microaerofílica se generó inflando la bolsa luego de retener la respiración por 20 seg, desinflándola e inflándola nuevamente (4 veces) y finalmente sellándola. Se aisló Campylobacter de 127 (96.2%) de las muestras incubadas en la jarra y de 129 (98%) de los especimenes incubados en la bolsa. La metodologia propuesta ofrece una posibilidad económica para los investigadores concientes de los costos de operación. |
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