Different methods of preparing rumen and caecum content from reindeer (Rangifer tarandus tarandus) for the analyses of minerals
Different methods were used for preparation of samples of rumen and caecum content from reindeer. The mineral contents of the samples were compared. Rumen and caecum samples from three reindeer were centrifuged at sampling and the fluid was stored frozen. Further preparation of these samples caused...
| Published in: | Rangifer |
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| Main Authors: | , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Septentrio Academic Publishing
1982
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| Subjects: | |
| Online Access: | https://doi.org/10.7557/2.2.2.413 https://doaj.org/article/978e3054debb462eb26e08d48bdd16b2 |
| Summary: | Different methods were used for preparation of samples of rumen and caecum content from reindeer. The mineral contents of the samples were compared. Rumen and caecum samples from three reindeer were centrifuged at sampling and the fluid was stored frozen. Further preparation of these samples caused a gradient in mineral concentration at centrifugation, varying between samples. This procedure consequently gave no reliable results. Hence, three methods of preparing total rumen content after freezing were compared: centrifugation, wet and dry ashing (after drying). Na, K, Mg, Ca, Mn, Fe, Cu and Zn were analysed. The mineral analyses of rumen fluid, 41 compared to total rumen content, gave divergent results for the different minerals, depending on solubility. Wet ashing and dry ashing gave approximately the same results. Considering these results, analyses of minerals in samples of total rumen or caecum content is recommended, rather than analyses in fluid samples. The samples should be frozen or dried immediately at collection, and later be used for either wet or dry ashing. Olika metoder vid preparering av vom- och blindtarmsinnhåll från ren (Rangifer tarandus tarandus) för analys av mineraler. Abstract in Swedish / Sammanfattning: Prov av vom- och blindtarmsinnehåll från ren preparerades på olika sätt och mineralåmnesinnehållet i proverna jämfördes. Vom- och blindtarmsprover från tre renar centrifugerades vid provtagningen och vätskan frystes. Vid vidare preparering av de fryslagrade vätskeproverna, uppstod vid centrifugering en gradient i fråga om mineralhalt. Denna var olika fôr olika prover. Detta tillvagagångssatt gav således inte tillförlitliga resultat. På grund härav jämfördes tre metoder fôr preparering av fryslagrat totalt vominnehåll: centrifugering samt våt- och torrföraskning (efter torkning). Na, K, Mg, Ca, Mn, Fe, Cu och Zn analyserades. Mineralanalyserna på vomvätska, jämfört med analyserna på totalt vominnehåll, gav ett fôr olika mineraler varierande resultat, beroende på olika löslighet. ... |
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