A Novel Method for Carbon Nanotube Functionalization Using Immobilized Candida antarctica Lipase

Carbon nanotubes (CNTs) have been proposed as nanovehicles for drug or antigen delivery since they can be functionalized with different biomolecules. For this purpose, different types of molecules have been chemically bonded to CNTs; however, this method has low efficiency and generates solvent wast...

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Published in:Nanomaterials
Main Authors: José Jesús Guzmán-Mendoza, David Chávez-Flores, Silvia Lorena Montes-Fonseca, Carmen González-Horta, Erasmo Orrantia-Borunda, Blanca Sánchez-Ramírez
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2022
Subjects:
Online Access:https://doi.org/10.3390/nano12091465
https://doaj.org/article/933c9bdf9510433b89cb9c01e72438f2
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spelling ftdoajarticles:oai:doaj.org/article:933c9bdf9510433b89cb9c01e72438f2 2023-05-15T13:39:43+02:00 A Novel Method for Carbon Nanotube Functionalization Using Immobilized Candida antarctica Lipase José Jesús Guzmán-Mendoza David Chávez-Flores Silvia Lorena Montes-Fonseca Carmen González-Horta Erasmo Orrantia-Borunda Blanca Sánchez-Ramírez 2022-04-01T00:00:00Z https://doi.org/10.3390/nano12091465 https://doaj.org/article/933c9bdf9510433b89cb9c01e72438f2 EN eng MDPI AG https://www.mdpi.com/2079-4991/12/9/1465 https://doaj.org/toc/2079-4991 doi:10.3390/nano12091465 2079-4991 https://doaj.org/article/933c9bdf9510433b89cb9c01e72438f2 Nanomaterials, Vol 12, Iss 1465, p 1465 (2022) carbon nanotubes enzymatic functionalization Candida antarctica lipase ester bond covalent functionalization Chemistry QD1-999 article 2022 ftdoajarticles https://doi.org/10.3390/nano12091465 2022-12-30T23:34:20Z Carbon nanotubes (CNTs) have been proposed as nanovehicles for drug or antigen delivery since they can be functionalized with different biomolecules. For this purpose, different types of molecules have been chemically bonded to CNTs; however, this method has low efficiency and generates solvent waste. Candida antarctica lipase is an enzyme that, in an organic solvent, can bind a carboxylic to a hydroxyl group by esterase activity. The objective of this work was to functionalize purified CNTs with insulin as a protein model using an immobilized lipase of Candida antarctica to develop a sustainable functionalization method with high protein attachment. The functionalized CNTs were characterized by scanning electron microscope (SEM), Raman spectroscopy, Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE). The enzymatic functionalization of insulin on the surface of the CNTs was found to have an efficiency of 21%, which is higher in conversion and greener than previously reported by the diimide-activated amidation method. These results suggest that enzymatic esterification is a convenient and efficient method for CNT functionalization with proteins. Moreover, this functionalization method can be used to enhance the cellular-specific release of proteins by lysosomal esterases. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Nanomaterials 12 9 1465
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic carbon nanotubes
enzymatic functionalization
Candida antarctica lipase
ester bond
covalent functionalization
Chemistry
QD1-999
spellingShingle carbon nanotubes
enzymatic functionalization
Candida antarctica lipase
ester bond
covalent functionalization
Chemistry
QD1-999
José Jesús Guzmán-Mendoza
David Chávez-Flores
Silvia Lorena Montes-Fonseca
Carmen González-Horta
Erasmo Orrantia-Borunda
Blanca Sánchez-Ramírez
A Novel Method for Carbon Nanotube Functionalization Using Immobilized Candida antarctica Lipase
topic_facet carbon nanotubes
enzymatic functionalization
Candida antarctica lipase
ester bond
covalent functionalization
Chemistry
QD1-999
description Carbon nanotubes (CNTs) have been proposed as nanovehicles for drug or antigen delivery since they can be functionalized with different biomolecules. For this purpose, different types of molecules have been chemically bonded to CNTs; however, this method has low efficiency and generates solvent waste. Candida antarctica lipase is an enzyme that, in an organic solvent, can bind a carboxylic to a hydroxyl group by esterase activity. The objective of this work was to functionalize purified CNTs with insulin as a protein model using an immobilized lipase of Candida antarctica to develop a sustainable functionalization method with high protein attachment. The functionalized CNTs were characterized by scanning electron microscope (SEM), Raman spectroscopy, Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE). The enzymatic functionalization of insulin on the surface of the CNTs was found to have an efficiency of 21%, which is higher in conversion and greener than previously reported by the diimide-activated amidation method. These results suggest that enzymatic esterification is a convenient and efficient method for CNT functionalization with proteins. Moreover, this functionalization method can be used to enhance the cellular-specific release of proteins by lysosomal esterases.
format Article in Journal/Newspaper
author José Jesús Guzmán-Mendoza
David Chávez-Flores
Silvia Lorena Montes-Fonseca
Carmen González-Horta
Erasmo Orrantia-Borunda
Blanca Sánchez-Ramírez
author_facet José Jesús Guzmán-Mendoza
David Chávez-Flores
Silvia Lorena Montes-Fonseca
Carmen González-Horta
Erasmo Orrantia-Borunda
Blanca Sánchez-Ramírez
author_sort José Jesús Guzmán-Mendoza
title A Novel Method for Carbon Nanotube Functionalization Using Immobilized Candida antarctica Lipase
title_short A Novel Method for Carbon Nanotube Functionalization Using Immobilized Candida antarctica Lipase
title_full A Novel Method for Carbon Nanotube Functionalization Using Immobilized Candida antarctica Lipase
title_fullStr A Novel Method for Carbon Nanotube Functionalization Using Immobilized Candida antarctica Lipase
title_full_unstemmed A Novel Method for Carbon Nanotube Functionalization Using Immobilized Candida antarctica Lipase
title_sort novel method for carbon nanotube functionalization using immobilized candida antarctica lipase
publisher MDPI AG
publishDate 2022
url https://doi.org/10.3390/nano12091465
https://doaj.org/article/933c9bdf9510433b89cb9c01e72438f2
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_source Nanomaterials, Vol 12, Iss 1465, p 1465 (2022)
op_relation https://www.mdpi.com/2079-4991/12/9/1465
https://doaj.org/toc/2079-4991
doi:10.3390/nano12091465
2079-4991
https://doaj.org/article/933c9bdf9510433b89cb9c01e72438f2
op_doi https://doi.org/10.3390/nano12091465
container_title Nanomaterials
container_volume 12
container_issue 9
container_start_page 1465
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