Viability and molecular authentication of Coccidioides immitis strains from culture collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil Viabilidade e autenticação molecular de cepas de Coccidioides immitis da coleção de culturas do Instituto Oswaldo Cruz, Rio de Janeiro, Brasil

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: Claudia C.F. Bezerra, Renata F. de Lima, Márcia S. Lazera, Bodo Wanke, Cíntia M. Borba
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2006
• Subjects: Coccidioides immitis; Preservação por longos períodos; PCR; Long-term preservation; Arctic medicine. Tropical medicine; RC955-962; Arctic; Sob’
• Online Access: https://doi.org/10.1590/S0037-86822006000300002; https://doaj.org/article/8e8a48f20f044cfdbc551449d871e106

Twenty Coccidioides immitis strains were evaluated. Only 5 of the 20 strains kept under mineral oil maintained their viability while all 5 subcultures preserved in water remained viable and none of the 13 subcultures kept in soil were viable. A 519 bp PCR product from the csa gene confirmed the iden...