Viability and molecular authentication of Coccidioides immitis strains from culture collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil Viabilidade e autenticação molecular de cepas de Coccidioides immitis da coleção de culturas do Instituto Oswaldo Cruz, Rio de Janeiro, Brasil

Twenty Coccidioides immitis strains were evaluated. Only 5 of the 20 strains kept under mineral oil maintained their viability while all 5 subcultures preserved in water remained viable and none of the 13 subcultures kept in soil were viable. A 519 bp PCR product from the csa gene confirmed the iden...

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Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical
Main Authors: Claudia C.F. Bezerra, Renata F. de Lima, Márcia S. Lazera, Bodo Wanke, Cíntia M. Borba
Format: Article in Journal/Newspaper
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2006
Subjects:
Coccidioides immitis
Preservação por longos períodos
PCR
Long-term preservation
Arctic medicine. Tropical medicine
RC955-962
Arctic
Sob’
Online Access:https://doi.org/10.1590/S0037-86822006000300002
https://doaj.org/article/8e8a48f20f044cfdbc551449d871e106
Description
Summary:Twenty Coccidioides immitis strains were evaluated. Only 5 of the 20 strains kept under mineral oil maintained their viability while all 5 subcultures preserved in water remained viable and none of the 13 subcultures kept in soil were viable. A 519 bp PCR product from the csa gene confirmed the identity of the strains. Vinte cepas de Coccidioides immitis foram avaliadas. Cinco das 20 cepas preservadas sob óleo mineral mantiveram-se viáveis, todas as 5 subculturas preservadas em água permaneceram viáveis e nenhuma das 13 subculturas mantidas em solo foi viável. Um produto de PCR de 519 pb do gene csa confirmou a identidade das cepas.

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