High-Throughput Microsatellite Marker Development for the Distylous Herb Primula mistassinica (Primulaceae)

• Published in: Applications in Plant Sciences
• Main Authors: Hannah Matheny, Joan Edwards, Luana S. Maroja
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2013
• Subjects: 454 sequencing; arctic-alpine plant; microsatellite enrichment; polymorphism; Primula mistassinica; Primulaceae; Biology (General); QH301-705.5; Botany; QK1-989; Arctic; Climate change
• Online Access: https://doi.org/10.3732/apps.1300002; https://doaj.org/article/8d055773734946e7bb5ff733f7230a5f

Premise of the study: Twelve microsatellite markers were developed for Primula mistassinica , a distylous, diploid arctic-alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species. Methods and Results: We used Roche/454 high-throughput technology to sequence microsatellite-enriched regions in the P. mistassinica genome. We developed 12 polymorphic microsatellite primer sets. These loci contained di-, tri-, and tetranucleotide repeats with two to nine alleles per locus when assessed in 23 individuals. Conclusions: Understanding the historical movements of P. mistassinica will provide insight to the survival prospects of current Arctic plant populations, which face the pressures of global, anthropogenic climate change.