High-Throughput Microsatellite Marker Development for the Distylous Herb Primula mistassinica (Primulaceae)

Premise of the study: Twelve microsatellite markers were developed for Primula mistassinica , a distylous, diploid arctic-alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species. M...

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Bibliographic Details
Published in:Applications in Plant Sciences
Main Authors: Hannah Matheny, Joan Edwards, Luana S. Maroja
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2013
Subjects:
454 sequencing
arctic-alpine plant
microsatellite enrichment
polymorphism
Primula mistassinica
Primulaceae
Biology (General)
QH301-705.5
Botany
QK1-989
Arctic
Climate change
Online Access:https://doi.org/10.3732/apps.1300002
https://doaj.org/article/8d055773734946e7bb5ff733f7230a5f
Description
Summary:Premise of the study: Twelve microsatellite markers were developed for Primula mistassinica , a distylous, diploid arctic-alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species. Methods and Results: We used Roche/454 high-throughput technology to sequence microsatellite-enriched regions in the P. mistassinica genome. We developed 12 polymorphic microsatellite primer sets. These loci contained di-, tri-, and tetranucleotide repeats with two to nine alleles per locus when assessed in 23 individuals. Conclusions: Understanding the historical movements of P. mistassinica will provide insight to the survival prospects of current Arctic plant populations, which face the pressures of global, anthropogenic climate change.

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