High-Throughput Microsatellite Marker Development for the Distylous Herb Primula mistassinica (Primulaceae)
Premise of the study: Twelve microsatellite markers were developed for Primula mistassinica , a distylous, diploid arctic-alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species. M...
Published in: | Applications in Plant Sciences |
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Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2013
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Subjects: | |
Online Access: | https://doi.org/10.3732/apps.1300002 https://doaj.org/article/8d055773734946e7bb5ff733f7230a5f |
Summary: | Premise of the study: Twelve microsatellite markers were developed for Primula mistassinica , a distylous, diploid arctic-alpine plant. The markers will be used to investigate the landscape genetics of a disjunct population on Isle Royale, Michigan, and the phylogeographic patterns of the species. Methods and Results: We used Roche/454 high-throughput technology to sequence microsatellite-enriched regions in the P. mistassinica genome. We developed 12 polymorphic microsatellite primer sets. These loci contained di-, tri-, and tetranucleotide repeats with two to nine alleles per locus when assessed in 23 individuals. Conclusions: Understanding the historical movements of P. mistassinica will provide insight to the survival prospects of current Arctic plant populations, which face the pressures of global, anthropogenic climate change. |
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