Analysis of molecular biology techniques for the diagnosis of human papillomavirus infection and cervical cancer prevention Análise de técnicas de biologia molecular para o diagnóstico de infecções causadas por papilomavírus humanos e a prevenção do câncer cervical

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: Fernanda Nahoum Carestiato, Kátia Cristina da Silva, Daniela Signorelli Balthazar, Licínio Silva, Marisa Marinho, Ledy Horto dos Santos Oliveira, Silvia Maria Baeta Cavalcanti
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2006
• Subjects: Papilomavírus humanos; Neoplasia intraepitelial escamosa; Câncer; Captura do híbrido; Reação em cadeia da polimerase; Human papillomavirus; Squamous intraepithelial neoplasia; Cancer; Hybrid capture; Polymerase chain reaction; Arctic medicine. Tropical medicine; RC955-962; Arctic
• Online Access: https://doi.org/10.1590/S0037-86822006000500002; https://doaj.org/article/8b96b5239ec44606acda41c60ee4cf0d

The objective of the present study was to evaluate the usefulness of molecular methodologies to access human papillomavirus genome in the genital tract. Samples from 136 women aged 17 to 52 years old obtained from the Dr. Sérgio Franco Laboratories between 2000 and 2001, were analyzed by the hybrid capture assay and amplified by PCR with generic primers MY09/MY11 and specific primers for types 16, 18, 31, 33, 35, 58. Viral genome was detected in 71.3% of the samples by hybrid capture and 75% by amplification. When cytopathology was used as a reference method for screening lesions, hybrid capture (p=0) and amplification (p=0.002) presented positive association. The 3 methods showed absolute agreement when cytopathology confirmed papillomavirus infection and high grade intraepithelial lesion. Disagreements occurred for 10 cases: seven inflammatory cases positive by PCR and negative for hybrid capture and 3 low squamous intraepithelial lesions positive for hybrid capture but negative for amplification. In conclusion, hybrid capture was shown to be sensitive and specific enough for use in clinical routines. Moreover, the evaluation of viral load values obtained by this method were shown to be related to the severity of the lesion and merit further studies to analyze the possible association with risk of progression to malignancy. O objetivo do nosso trabalho foi avaliar o emprego de métodos moleculares para comprovar a presença dos papilomavírus humanos no trato genital. Amostras de 136 pacientes com idades entre 17 e 52 anos, coletadas nos Laboratórios Dr. Sérgio Franco entre 2000 e 2001, foram analisadas pelas técnicas de captura híbrida e amplificação pela reação em cadeia da polimerase com primers genéricos MY09/MY11 e específicos para os tipos 16, 18, 31, 33, 35, 58. O genoma viral foi detectado em 71,3% dessas amostras pela captura híbrida e 75% pela reação em cadeia da polimerase. Quando a citopatologia foi usada como método de referência para rastreamento das lesões, a captura (p=0) e a amplificação ...