Purification of a 19-kDa pore-forming cytolysin from the sea anemone Heteractis magnifica
Pore-forming cytolysins of 19 kDa from sea anemones present a remarkable cytolytic property. In the present work, a purified 19-kDa cytolysin was obtained from the sea anemone Heteractis magnifica. The purification steps involved ammonium sulfate precipitation and subsequently desalting by dialysis...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
SciELO
2010
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Subjects: | |
Online Access: | https://doi.org/10.1590/S1678-91992010000300019 https://doaj.org/article/8b406b5983dd4b9090da6528a4fb4695 |
Summary: | Pore-forming cytolysins of 19 kDa from sea anemones present a remarkable cytolytic property. In the present work, a purified 19-kDa cytolysin was obtained from the sea anemone Heteractis magnifica. The purification steps involved ammonium sulfate precipitation and subsequently desalting by dialysis against 10 mM sodium phosphate buffer (pH 7.4), followed by anion exchange chromatography in DEAE-Sepharose® column (GE Healthcare, Sweden) and gel filtration chromatography using Sephadex® G-50 matrix (GE Healthcare, Sweden). The active fractions from the gel filtration chromatography were pooled and rechromatographed in the same column. The final active fraction showed a prominent protein band of molecular mass of 19 kDa when analyzed by SDS-PAGE. |
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