Draft whole genome sequence for four highly copper resistant soil isolates Pseudomonas lactis strain UKR1, Pseudomonas panacis strain UKR2, and Pseudomonas veronii strains UKR3 and UKR4
Environmental copper pollution causes major destruction to ecological systems, which require the development of environmentally friendly biotechnological, in particular, microbial methods for copper removal. These methods rely on the availability of microorganisms resistant to high levels of copper....
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ftdoajarticles:oai:doaj.org/article:870ca520027f4480b111677f209d4f5d 2023-05-15T13:51:08+02:00 Draft whole genome sequence for four highly copper resistant soil isolates Pseudomonas lactis strain UKR1, Pseudomonas panacis strain UKR2, and Pseudomonas veronii strains UKR3 and UKR4 Olesia Havryliuk Vira Hovorukha Marianna Patrauchan Noha H. Youssef Oleksandr Tashyrev 2020-09-01T00:00:00Z https://doi.org/10.1016/j.crmicr.2020.06.002 https://doaj.org/article/870ca520027f4480b111677f209d4f5d EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2666517420300067 https://doaj.org/toc/2666-5174 2666-5174 doi:10.1016/j.crmicr.2020.06.002 https://doaj.org/article/870ca520027f4480b111677f209d4f5d Current Research in Microbial Sciences, Vol 1, Iss , Pp 44-52 (2020) Сopper-resistant microorganisms Environmental biotechnologies Soil isolates Arctic Antarctic and Ukrainian soils Microbiology QR1-502 Genetics QH426-470 article 2020 ftdoajarticles https://doi.org/10.1016/j.crmicr.2020.06.002 2022-12-31T04:22:34Z Environmental copper pollution causes major destruction to ecological systems, which require the development of environmentally friendly biotechnological, in particular, microbial methods for copper removal. These methods rely on the availability of microorganisms resistant to high levels of copper. Here we isolated four bacterial strains with record resistance to up to 1.0 M Cu(II). The strains were isolated from ecologically diverse soil samples, and their genomes were sequenced. A 16S rRNA sequence-based phylogenetic analysis identified that all four isolates belong to the genus Pseudomonas. Particularly, strains UKR1 and UKR2 isolated from Kyiv region in Ukraine were identified as P. lactis and P. panacis, respectively, and strains UKR3 and UKR4 isolated from Svalbard Island in the Arctic Ocean and Galindez Island in Antarctica, respectively, were identified as P. veronii. Initial in-silico screening for genes encoding copper resistance mechanisms showed that all four strains encode copper resistance proteins CopA, CopB, CopD, CopA3, CopZ, as well as two-component regulatory system CusRS, all known to be associated with metal resistance in Pseudomonas genus. Further detailed studies will aim to characterize the full genomic potential of the isolates to enable their application for copper bioremediation in contaminated soils and industrial wastewaters. Article in Journal/Newspaper Antarc* Antarctic Antarctica Arctic Arctic Ocean Galindez Island Svalbard Directory of Open Access Journals: DOAJ Articles Antarctic Arctic Arctic Ocean Galindez ENVELOPE(-64.248,-64.248,-65.250,-65.250) Galindez Island ENVELOPE(-64.267,-64.267,-65.250,-65.250) Svalbard Current Research in Microbial Sciences 1 44 52 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Сopper-resistant microorganisms Environmental biotechnologies Soil isolates Arctic Antarctic and Ukrainian soils Microbiology QR1-502 Genetics QH426-470 |
spellingShingle |
Сopper-resistant microorganisms Environmental biotechnologies Soil isolates Arctic Antarctic and Ukrainian soils Microbiology QR1-502 Genetics QH426-470 Olesia Havryliuk Vira Hovorukha Marianna Patrauchan Noha H. Youssef Oleksandr Tashyrev Draft whole genome sequence for four highly copper resistant soil isolates Pseudomonas lactis strain UKR1, Pseudomonas panacis strain UKR2, and Pseudomonas veronii strains UKR3 and UKR4 |
topic_facet |
Сopper-resistant microorganisms Environmental biotechnologies Soil isolates Arctic Antarctic and Ukrainian soils Microbiology QR1-502 Genetics QH426-470 |
description |
Environmental copper pollution causes major destruction to ecological systems, which require the development of environmentally friendly biotechnological, in particular, microbial methods for copper removal. These methods rely on the availability of microorganisms resistant to high levels of copper. Here we isolated four bacterial strains with record resistance to up to 1.0 M Cu(II). The strains were isolated from ecologically diverse soil samples, and their genomes were sequenced. A 16S rRNA sequence-based phylogenetic analysis identified that all four isolates belong to the genus Pseudomonas. Particularly, strains UKR1 and UKR2 isolated from Kyiv region in Ukraine were identified as P. lactis and P. panacis, respectively, and strains UKR3 and UKR4 isolated from Svalbard Island in the Arctic Ocean and Galindez Island in Antarctica, respectively, were identified as P. veronii. Initial in-silico screening for genes encoding copper resistance mechanisms showed that all four strains encode copper resistance proteins CopA, CopB, CopD, CopA3, CopZ, as well as two-component regulatory system CusRS, all known to be associated with metal resistance in Pseudomonas genus. Further detailed studies will aim to characterize the full genomic potential of the isolates to enable their application for copper bioremediation in contaminated soils and industrial wastewaters. |
format |
Article in Journal/Newspaper |
author |
Olesia Havryliuk Vira Hovorukha Marianna Patrauchan Noha H. Youssef Oleksandr Tashyrev |
author_facet |
Olesia Havryliuk Vira Hovorukha Marianna Patrauchan Noha H. Youssef Oleksandr Tashyrev |
author_sort |
Olesia Havryliuk |
title |
Draft whole genome sequence for four highly copper resistant soil isolates Pseudomonas lactis strain UKR1, Pseudomonas panacis strain UKR2, and Pseudomonas veronii strains UKR3 and UKR4 |
title_short |
Draft whole genome sequence for four highly copper resistant soil isolates Pseudomonas lactis strain UKR1, Pseudomonas panacis strain UKR2, and Pseudomonas veronii strains UKR3 and UKR4 |
title_full |
Draft whole genome sequence for four highly copper resistant soil isolates Pseudomonas lactis strain UKR1, Pseudomonas panacis strain UKR2, and Pseudomonas veronii strains UKR3 and UKR4 |
title_fullStr |
Draft whole genome sequence for four highly copper resistant soil isolates Pseudomonas lactis strain UKR1, Pseudomonas panacis strain UKR2, and Pseudomonas veronii strains UKR3 and UKR4 |
title_full_unstemmed |
Draft whole genome sequence for four highly copper resistant soil isolates Pseudomonas lactis strain UKR1, Pseudomonas panacis strain UKR2, and Pseudomonas veronii strains UKR3 and UKR4 |
title_sort |
draft whole genome sequence for four highly copper resistant soil isolates pseudomonas lactis strain ukr1, pseudomonas panacis strain ukr2, and pseudomonas veronii strains ukr3 and ukr4 |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://doi.org/10.1016/j.crmicr.2020.06.002 https://doaj.org/article/870ca520027f4480b111677f209d4f5d |
long_lat |
ENVELOPE(-64.248,-64.248,-65.250,-65.250) ENVELOPE(-64.267,-64.267,-65.250,-65.250) |
geographic |
Antarctic Arctic Arctic Ocean Galindez Galindez Island Svalbard |
geographic_facet |
Antarctic Arctic Arctic Ocean Galindez Galindez Island Svalbard |
genre |
Antarc* Antarctic Antarctica Arctic Arctic Ocean Galindez Island Svalbard |
genre_facet |
Antarc* Antarctic Antarctica Arctic Arctic Ocean Galindez Island Svalbard |
op_source |
Current Research in Microbial Sciences, Vol 1, Iss , Pp 44-52 (2020) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2666517420300067 https://doaj.org/toc/2666-5174 2666-5174 doi:10.1016/j.crmicr.2020.06.002 https://doaj.org/article/870ca520027f4480b111677f209d4f5d |
op_doi |
https://doi.org/10.1016/j.crmicr.2020.06.002 |
container_title |
Current Research in Microbial Sciences |
container_volume |
1 |
container_start_page |
44 |
op_container_end_page |
52 |
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1766254745876430848 |