Draft whole genome sequence for four highly copper resistant soil isolates Pseudomonas lactis strain UKR1, Pseudomonas panacis strain UKR2, and Pseudomonas veronii strains UKR3 and UKR4

Environmental copper pollution causes major destruction to ecological systems, which require the development of environmentally friendly biotechnological, in particular, microbial methods for copper removal. These methods rely on the availability of microorganisms resistant to high levels of copper....

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Bibliographic Details
Published in:Current Research in Microbial Sciences
Main Authors: Olesia Havryliuk, Vira Hovorukha, Marianna Patrauchan, Noha H. Youssef, Oleksandr Tashyrev
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2020
Subjects:
Сopper-resistant microorganisms
Environmental biotechnologies
Soil isolates
Arctic
Antarctic
and Ukrainian soils
Microbiology
QR1-502
Genetics
QH426-470
Arctic Ocean
Galindez
Galindez Island
Svalbard
Antarc*
Antarctica
Online Access:https://doi.org/10.1016/j.crmicr.2020.06.002
https://doaj.org/article/870ca520027f4480b111677f209d4f5d
Description
Summary:Environmental copper pollution causes major destruction to ecological systems, which require the development of environmentally friendly biotechnological, in particular, microbial methods for copper removal. These methods rely on the availability of microorganisms resistant to high levels of copper. Here we isolated four bacterial strains with record resistance to up to 1.0 M Cu(II). The strains were isolated from ecologically diverse soil samples, and their genomes were sequenced. A 16S rRNA sequence-based phylogenetic analysis identified that all four isolates belong to the genus Pseudomonas. Particularly, strains UKR1 and UKR2 isolated from Kyiv region in Ukraine were identified as P. lactis and P. panacis, respectively, and strains UKR3 and UKR4 isolated from Svalbard Island in the Arctic Ocean and Galindez Island in Antarctica, respectively, were identified as P. veronii. Initial in-silico screening for genes encoding copper resistance mechanisms showed that all four strains encode copper resistance proteins CopA, CopB, CopD, CopA3, CopZ, as well as two-component regulatory system CusRS, all known to be associated with metal resistance in Pseudomonas genus. Further detailed studies will aim to characterize the full genomic potential of the isolates to enable their application for copper bioremediation in contaminated soils and industrial wastewaters.

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