Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway
Objective: To investigate the effect of memantine on Parkinson’s disease cell models. Methods: Parkinson’s disease cell models were established using PC12 cells incubated with 6-hydroxydopamine (6-OHDA). Flow cytometry and microscopy were used to investigate the apoptotic process and the percentage...
Published in: | Asian Pacific Journal of Tropical Medicine |
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ftdoajarticles:oai:doaj.org/article:84992b8131484affaf1072c72c96c670 2023-05-15T15:04:00+02:00 Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway Wei Wu Rui Wang Yong-Mei Fu Jie Zhou Hai-Chao Huang Wei-Wang Gu 2019-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.271981 https://doaj.org/article/84992b8131484affaf1072c72c96c670 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=14;spage=59;epage=68;aulast=Wu https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.271981 https://doaj.org/article/84992b8131484affaf1072c72c96c670 Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 14, Pp 59-68 (2019) parkinson’s disease 6-ohda nur77 memantine apoptosis Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.4103/1995-7645.271981 2022-12-31T12:26:00Z Objective: To investigate the effect of memantine on Parkinson’s disease cell models. Methods: Parkinson’s disease cell models were established using PC12 cells incubated with 6-hydroxydopamine (6-OHDA). Flow cytometry and microscopy were used to investigate the apoptotic process and the percentage of different apoptotic stages. PC12 cells were infected with lentiviral vectors to knockdown Nur77. Western blotting was used to detect the expression of Nur77 and caspase -3, -8, -9, -12 in PC12 cells withdifferent concentrations of 6-OHDA or 6-OHDA+memantine. Results: 6-OHDA led to apoptosis PC12 cells, and increased the expression of Nur77 and caspases. Memantine significantly inhibited 6-OHDA-induced apoptosis of PC12 cells. Meanwhile, memantine could mitigate apoptosis of PC12 cells by regulating the Nur77 and caspase pathway. Conclusions: Memantine has a protective effect on the PC12 cell model via regulating the Nur77 and caspases pathway. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 12 14 59 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
parkinson’s disease 6-ohda nur77 memantine apoptosis Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
parkinson’s disease 6-ohda nur77 memantine apoptosis Arctic medicine. Tropical medicine RC955-962 Wei Wu Rui Wang Yong-Mei Fu Jie Zhou Hai-Chao Huang Wei-Wang Gu Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway |
topic_facet |
parkinson’s disease 6-ohda nur77 memantine apoptosis Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To investigate the effect of memantine on Parkinson’s disease cell models. Methods: Parkinson’s disease cell models were established using PC12 cells incubated with 6-hydroxydopamine (6-OHDA). Flow cytometry and microscopy were used to investigate the apoptotic process and the percentage of different apoptotic stages. PC12 cells were infected with lentiviral vectors to knockdown Nur77. Western blotting was used to detect the expression of Nur77 and caspase -3, -8, -9, -12 in PC12 cells withdifferent concentrations of 6-OHDA or 6-OHDA+memantine. Results: 6-OHDA led to apoptosis PC12 cells, and increased the expression of Nur77 and caspases. Memantine significantly inhibited 6-OHDA-induced apoptosis of PC12 cells. Meanwhile, memantine could mitigate apoptosis of PC12 cells by regulating the Nur77 and caspase pathway. Conclusions: Memantine has a protective effect on the PC12 cell model via regulating the Nur77 and caspases pathway. |
format |
Article in Journal/Newspaper |
author |
Wei Wu Rui Wang Yong-Mei Fu Jie Zhou Hai-Chao Huang Wei-Wang Gu |
author_facet |
Wei Wu Rui Wang Yong-Mei Fu Jie Zhou Hai-Chao Huang Wei-Wang Gu |
author_sort |
Wei Wu |
title |
Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway |
title_short |
Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway |
title_full |
Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway |
title_fullStr |
Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway |
title_full_unstemmed |
Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway |
title_sort |
memantine inhibits 6-ohda-induced apoptosis pc12 cells via the nurr77 and caspase pathway |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/1995-7645.271981 https://doaj.org/article/84992b8131484affaf1072c72c96c670 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 14, Pp 59-68 (2019) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=14;spage=59;epage=68;aulast=Wu https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.271981 https://doaj.org/article/84992b8131484affaf1072c72c96c670 |
op_doi |
https://doi.org/10.4103/1995-7645.271981 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
12 |
container_issue |
14 |
container_start_page |
59 |
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1766335834677575680 |