Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway

Objective: To investigate the effect of memantine on Parkinson’s disease cell models. Methods: Parkinson’s disease cell models were established using PC12 cells incubated with 6-hydroxydopamine (6-OHDA). Flow cytometry and microscopy were used to investigate the apoptotic process and the percentage...

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Bibliographic Details
Published in:Asian Pacific Journal of Tropical Medicine
Main Authors: Wei Wu, Rui Wang, Yong-Mei Fu, Jie Zhou, Hai-Chao Huang, Wei-Wang Gu
Format: Article in Journal/Newspaper
Language:English
Published: Wolters Kluwer Medknow Publications 2019
Subjects:
parkinson’s disease
6-ohda
nur77
memantine
apoptosis
Arctic medicine. Tropical medicine
RC955-962
Arctic
Online Access:https://doi.org/10.4103/1995-7645.271981
https://doaj.org/article/84992b8131484affaf1072c72c96c670
Description
Summary:Objective: To investigate the effect of memantine on Parkinson’s disease cell models. Methods: Parkinson’s disease cell models were established using PC12 cells incubated with 6-hydroxydopamine (6-OHDA). Flow cytometry and microscopy were used to investigate the apoptotic process and the percentage of different apoptotic stages. PC12 cells were infected with lentiviral vectors to knockdown Nur77. Western blotting was used to detect the expression of Nur77 and caspase -3, -8, -9, -12 in PC12 cells withdifferent concentrations of 6-OHDA or 6-OHDA+memantine. Results: 6-OHDA led to apoptosis PC12 cells, and increased the expression of Nur77 and caspases. Memantine significantly inhibited 6-OHDA-induced apoptosis of PC12 cells. Meanwhile, memantine could mitigate apoptosis of PC12 cells by regulating the Nur77 and caspase pathway. Conclusions: Memantine has a protective effect on the PC12 cell model via regulating the Nur77 and caspases pathway.

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