Entamoeba histolytica: fecal antigen capture immunoassay for the diagnosis of enteric amebiasis by a monoclonal antibody Entamoeba histolytica: imunodiagnóstico, através de captura de antígeno fecal em amebíase entérica por um anticorpo monoclonal
Amebiasis continues to be of epidemiological importance in underdeveloped countries. Clinical diagnosis and epidemiological setting in a region are based on the fecal microscopic identification of cysts or trophozoites. This procedure requires well trained personnel, is laborious, of low sensitivity...
| Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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| Main Authors: | , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Universidade de São Paulo (USP)
1996
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| Subjects: | |
| Online Access: | https://doi.org/10.1590/S0036-46651996000100008 https://doaj.org/article/8398e81e769542558bf262f1a284dc07 |
| Summary: | Amebiasis continues to be of epidemiological importance in underdeveloped countries. Clinical diagnosis and epidemiological setting in a region are based on the fecal microscopic identification of cysts or trophozoites. This procedure requires well trained personnel, is laborious, of low sensitivity and frequently yields false-positives results. The present study was designed to develop an immuno-enzymatic fecal 96 kDa antigen capture test (COPROELISA-Eh) more sensitive and specific than microscopic diagnosis of amebiasis. Triplicates of 177 stool samples processed by the formol-ether concentration method, were defined as positive or negative by three experienced microscopic observers. Another aliquot was submitted to the antigen capture test by a monoclonal antibody against a specific membrane antigen of pathogenic strains of Entamoeba histolytica. Optical densities were interpreted as positive when they exceeded the mean value of negative samples plus two standard deviations. COPROELISA-Eh showed a 94.4% sensitivity, 98.3% specificity, 96.2% positive predictive value and 97.6% negative predictive value for the detection of E. histolytica in feces. COPROELISA-Eh is more sensitive and specific than microscopic examination, does not require specially trained personnel and allows the simultaneous processing of a large number of samples. A amebíase mantém sua importância epidemiológica em países subdesenvolvidos onde sua prevalência a converteu na parasitose de maior morbidade e mortalidade após malaria e esquistosomose. Em regra, tanto o diagnóstico clínico como os levantamentos epidemiológicos assentam na identificação microscópica de cistos e/ou trofozoítos em extractos fecais. Este procedimento requer pessoal muito bem treinado, é laborioso, e frequentemente fornece resultados contraditórios. Para obviar estas dificuldades, no presente trabalho montamos uma técnica de diagnóstico imunoenzimático baseado na captura de um antígeno de 96 kDa presente nas fezes de indivíduos infectados pela E. hystolytica ... |
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