Concentration and purification by magnetic separation of the erythrocytic stages of all human Plasmodium species

Abstract Background Parasite concentration methods facilitate molecular, biochemical and immunological research on the erythrocytic stages of Plasmodium . In this paper, an adaptation of magnetic MACS ® columns for the purification of human Plasmodium species is presented. This method was useful for...

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Bibliographic Details
Published in:Malaria Journal
Main Authors: Benoit-Vical Françoise, Nepveu Françoise, Parzy Daniel, Morlais Isabelle, Reybier Karine, Chevalley Séverine, Berry Antoine, Ribaut Clotilde, Valentin Alexis
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2008
Subjects:
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1186/1475-2875-7-45
https://doaj.org/article/83089795869d469884bc2ee977381d4f
Description
Summary:Abstract Background Parasite concentration methods facilitate molecular, biochemical and immunological research on the erythrocytic stages of Plasmodium . In this paper, an adaptation of magnetic MACS ® columns for the purification of human Plasmodium species is presented. This method was useful for the concentration/purification of either schizonts or gametocytes. Results and conclusions The magnetic removal of non-parasitized red blood cells (in vivo and in vitro) using magnetic columns (MACS) was evaluated. This easy-to-use technique enriched schizonts and gametocytes from Plasmodium falciparum in vitro cultures with a very high degree of purity. In addition, all haemozoin-containing stages (schizonts and/or gametocytes) from the peripheral blood of infected patients could be concentrated using this method. This method is particularly useful for the concentration of non-falciparum species, which do not grow in culture and are otherwise difficult to obtain in large amounts.

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