Molecular characterization of nucleoprotein gene of rabies virus from Maharashtra, India

Context: Rabies poses a serious public health concern in developing countries such as India. Aims: The study focuses on molecular diagnosis of street rabies virus (RABV) from human clinical specimens received from Maharashtra, India. Materials and Methods: Nucleoprotein gene from eight (of total 20...

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Bibliographic Details
Published in:Journal of Postgraduate Medicine
Main Authors: S Mehta, P Charan, R Dahake, S Mukherjee, A Chowdhary
Format: Article in Journal/Newspaper
Language:English
Published: Wolters Kluwer Medknow Publications 2016
Subjects:
Epidemiology
nucleoprotein gene
phylogenetic analysis
rabies
Medicine
R
Arctic
Online Access:https://doi.org/10.4103/0022-3859.175006
https://doaj.org/article/824ced4173d642cdbdb7a6c2cb266cf4
Description
Summary:Context: Rabies poses a serious public health concern in developing countries such as India. Aims: The study focuses on molecular diagnosis of street rabies virus (RABV) from human clinical specimens received from Maharashtra, India. Materials and Methods: Nucleoprotein gene from eight (of total 20 suspected samples) rabies cases that tested positive for rabies antigen using reverse transcriptase-polymerase chain reaction (RT-PCR) were sequenced. Results: Sequence analysis using basic local alignment search tool (BLAST) and multiple sequence alignment (MSA) and phylogenetic analysis showed similarity to previously reported sequences from India and those of Arctic lineages. Conclusions: The circulating RABV strains in Maharashtra, India show genetic relatedness to RABV strains reported from Indo-Arctic lineages and India-South and Japan.

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