Can Aspergillus fumigatus conidia cause false-positive results in the galactomannan enzyme immunoassay test?

Abstract INTRODUCTION: Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated. METHODS: We studied...

Full description

Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical
Main Authors: Ângela Leitzke Cabana, Josiara Furtado Mendes, Gabriel Baracy Klafke, Tchana Martinez Brandolt, Aryse Martins Melo, Mário Carlos Araújo Meireles, Melissa Orzechowski Xavier
Format: Article in Journal/Newspaper
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT)
Subjects:
Invasive aspergillosis
Diagnosis
Platelia® Aspergillus EIA
Galactomannan
Air quality
Arctic medicine. Tropical medicine
RC955-962
Arctic
Eia
Online Access:https://doi.org/10.1590/0037-8682-0317-2017
https://doaj.org/article/810b56e06cc04dad96e8b183cbf63820
Description
Summary:Abstract INTRODUCTION: Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, the impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated. METHODS: We studied 12 A. fumigatus isolates. Fungal conidia were serially diluted and tested for GM detection using the Platelia® Aspergillus enzyme immunoassay (EIA). RESULTS: The conidia concentration required for an EIA-positive result was 4.8 × 103 (median). CONCLUSIONS: This is the first study to evaluate the impact of environmental contamination on the Platelia® Aspergillus EIA assay. Only massive contamination can interfere with GM optical readings, suggesting that environmental contamination does not cause false-positive test results.

Similar Items