In vitro digestion analysis of soft candy containing peptide-zinc chelates derived from low-fluoride protein hydrolysates of Antarctic krill (Euphausia superba) powder

This study developed a soft candy with peptide-zinc chelates using low-fluorine protein hydrolysates derived from Antarctic krill powder. To achieve this, the enzymatic hydrolysis conditions of the protein isolates were optimized through single-factor experiments. Protein hydrolysates were used to p...

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Bibliographic Details
Published in:Future Foods
Main Authors: Qinguo Quan, Hongwu Ji, Ming Chen, Zewei Zhang
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2024
Subjects:
Online Access:https://doi.org/10.1016/j.fufo.2024.100390
https://doaj.org/article/7ef64a69ad854a5a9e8821820cb89f9b
Description
Summary:This study developed a soft candy with peptide-zinc chelates using low-fluorine protein hydrolysates derived from Antarctic krill powder. To achieve this, the enzymatic hydrolysis conditions of the protein isolates were optimized through single-factor experiments. Protein hydrolysates were used to produce Antarctic krill peptides (AKP) through ceramic membrane microfiltration at various pressure levels. AKP-zinc chelates (AKP-Zn) were prepared by mixing AKP and zinc sulfate, subsequent to characterized the resulting complex. Finally, we examined the tolerance of AKP-Zn and soft candy containing AKP-Zn (SC-AKP-Zn) to simulated gastrointestinal digestion in vitro. The optimal enzyme mix was a 1:1 ratio of alkaline and flavored protease, with 4000 U/g enzymes, pH 7.5 and 3 h, and the ceramic membrane microfiltration increased the protein content in AKP by approximately 85.34 ± 3.54 %. Characterization showed that AKP effectively interacts zinc ions through bonding with oxygen and nitrogen atoms, helps the strength of AKP-Zn in various pH levels and simulated digestive systems. AKP-Zn and SC-AKP-Zn showed higher bioavailability compared to zinc sulfate and zinc gluconate. These results provide a solid theoretical foundation for creating ready-to-eat food products with AKP-Zn and offer new insights into the potential applications of Antarctic krill proteins.