EVALUATION OF A RAPID SCREENING ASSAY FOR BACTERIAL IDENTIFICATION (DOT-ELISA) IN FECAL SAMPLES FROM CHILDREN
With the objective of standardizing a Dot Enzyme-Linked Immunosorbent Assay (Dot-ELISA) to detect antigens of fecal bacterial enteropathogens, 250 children, aged under 36 months and of both sexes, were studied; of which 162 had acute gastroenteritis. The efficacy of a rapid screening assay for bacte...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
1997
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ftdoajarticles:oai:doaj.org/article:7b4c239cb72046678f85a549e0f09362 2024-09-09T19:28:01+00:00 EVALUATION OF A RAPID SCREENING ASSAY FOR BACTERIAL IDENTIFICATION (DOT-ELISA) IN FECAL SAMPLES FROM CHILDREN Etelvina BOCCATTO Sânia Alves dos SANTOS Neusa Augusta Oliveira MAZIERI Dahir Ramos de ANDRADE Francisco Roque CARRAZZA Roberto Mitio YANAGUITA 1997-01-01T00:00:00Z https://doi.org/10.1590/S0036-46651997000100005 https://doaj.org/article/7b4c239cb72046678f85a549e0f09362 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651997000100005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651997000100005 0036-4665 1678-9946 https://doaj.org/article/7b4c239cb72046678f85a549e0f09362 Revista do Instituto de Medicina Tropical de São Paulo, Vol 39, Iss 1, p 21 (1997) Infantile diarrhea Enteropathogens Fecal antigens Stool culture Dot-ELISA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1997 ftdoajarticles https://doi.org/10.1590/S0036-46651997000100005 2024-08-05T17:49:30Z With the objective of standardizing a Dot Enzyme-Linked Immunosorbent Assay (Dot-ELISA) to detect antigens of fecal bacterial enteropathogens, 250 children, aged under 36 months and of both sexes, were studied; of which 162 had acute gastroenteritis. The efficacy of a rapid screening assay for bacterial enteropathogens (enteropathogenic Escherichia coli "EPEC", enteroinvasive Escherichia coli "EIEC", Salmonella spp. and Shigella spp.) was evaluated. The fecal samples were also submitted to a traditional method of stool culture for comparison. The concordance index between the two techniques, calculated using the Kappa (k) index for the above mentioned bacterial strains was 0.8859, 0.9055, 0.7932 and 0.7829 respectively. These values express an almost perfect degree of concordance for the first two and substantial concordance for the latter two, thus enabling this technique to be applied in the early diagnosis of diarrhea in infants. With a view to increasing the sensitivity and specificity of this immunological test, a study was made of the antigenic preparations obtained from two types of treatment: 1) deproteinization by heating; 2) precipitation and concentration of the lipopolysaccharide antigen (LPS) using an ethanol-acetone solution, which was then heated in the presence of sodium EDTA Com o objetivo de padronizar um Dot Enzyme-Linked Immunosorbent Assay (Dot-ELISA) para a detecção de antígenos de enteropatógenos bacterianos fecais, estudaram-se 250 crianças, abaixo de 36 meses de idade, de ambos os sexos, 162 portadoras de gastroenterite aguda. Avaliou-se a eficácia de um teste rápido para bactérias enteropatógenas (Escherichia coli enteropatogênica "EPEC", Escherichia coli enteroinvasora" EIEC", Salmonella spp. e Shigella spp.). As amostras fecais foram também submetidas à metodologia tradicional de coprocultura para comparação. Os índices de concordância entre as 2 técnicas, calculado através do índice Kappa (k) para as cepas bacterianas foram 0,8859; 0,9055; 0,7932 e 0,7829 respectivamente. Estes ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Aguda ENVELOPE(-26.750,-26.750,-76.150,-76.150) Arctic Revista do Instituto de Medicina Tropical de São Paulo 39 1 21 28 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Infantile diarrhea Enteropathogens Fecal antigens Stool culture Dot-ELISA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Infantile diarrhea Enteropathogens Fecal antigens Stool culture Dot-ELISA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Etelvina BOCCATTO Sânia Alves dos SANTOS Neusa Augusta Oliveira MAZIERI Dahir Ramos de ANDRADE Francisco Roque CARRAZZA Roberto Mitio YANAGUITA EVALUATION OF A RAPID SCREENING ASSAY FOR BACTERIAL IDENTIFICATION (DOT-ELISA) IN FECAL SAMPLES FROM CHILDREN |
topic_facet |
Infantile diarrhea Enteropathogens Fecal antigens Stool culture Dot-ELISA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
With the objective of standardizing a Dot Enzyme-Linked Immunosorbent Assay (Dot-ELISA) to detect antigens of fecal bacterial enteropathogens, 250 children, aged under 36 months and of both sexes, were studied; of which 162 had acute gastroenteritis. The efficacy of a rapid screening assay for bacterial enteropathogens (enteropathogenic Escherichia coli "EPEC", enteroinvasive Escherichia coli "EIEC", Salmonella spp. and Shigella spp.) was evaluated. The fecal samples were also submitted to a traditional method of stool culture for comparison. The concordance index between the two techniques, calculated using the Kappa (k) index for the above mentioned bacterial strains was 0.8859, 0.9055, 0.7932 and 0.7829 respectively. These values express an almost perfect degree of concordance for the first two and substantial concordance for the latter two, thus enabling this technique to be applied in the early diagnosis of diarrhea in infants. With a view to increasing the sensitivity and specificity of this immunological test, a study was made of the antigenic preparations obtained from two types of treatment: 1) deproteinization by heating; 2) precipitation and concentration of the lipopolysaccharide antigen (LPS) using an ethanol-acetone solution, which was then heated in the presence of sodium EDTA Com o objetivo de padronizar um Dot Enzyme-Linked Immunosorbent Assay (Dot-ELISA) para a detecção de antígenos de enteropatógenos bacterianos fecais, estudaram-se 250 crianças, abaixo de 36 meses de idade, de ambos os sexos, 162 portadoras de gastroenterite aguda. Avaliou-se a eficácia de um teste rápido para bactérias enteropatógenas (Escherichia coli enteropatogênica "EPEC", Escherichia coli enteroinvasora" EIEC", Salmonella spp. e Shigella spp.). As amostras fecais foram também submetidas à metodologia tradicional de coprocultura para comparação. Os índices de concordância entre as 2 técnicas, calculado através do índice Kappa (k) para as cepas bacterianas foram 0,8859; 0,9055; 0,7932 e 0,7829 respectivamente. Estes ... |
format |
Article in Journal/Newspaper |
author |
Etelvina BOCCATTO Sânia Alves dos SANTOS Neusa Augusta Oliveira MAZIERI Dahir Ramos de ANDRADE Francisco Roque CARRAZZA Roberto Mitio YANAGUITA |
author_facet |
Etelvina BOCCATTO Sânia Alves dos SANTOS Neusa Augusta Oliveira MAZIERI Dahir Ramos de ANDRADE Francisco Roque CARRAZZA Roberto Mitio YANAGUITA |
author_sort |
Etelvina BOCCATTO |
title |
EVALUATION OF A RAPID SCREENING ASSAY FOR BACTERIAL IDENTIFICATION (DOT-ELISA) IN FECAL SAMPLES FROM CHILDREN |
title_short |
EVALUATION OF A RAPID SCREENING ASSAY FOR BACTERIAL IDENTIFICATION (DOT-ELISA) IN FECAL SAMPLES FROM CHILDREN |
title_full |
EVALUATION OF A RAPID SCREENING ASSAY FOR BACTERIAL IDENTIFICATION (DOT-ELISA) IN FECAL SAMPLES FROM CHILDREN |
title_fullStr |
EVALUATION OF A RAPID SCREENING ASSAY FOR BACTERIAL IDENTIFICATION (DOT-ELISA) IN FECAL SAMPLES FROM CHILDREN |
title_full_unstemmed |
EVALUATION OF A RAPID SCREENING ASSAY FOR BACTERIAL IDENTIFICATION (DOT-ELISA) IN FECAL SAMPLES FROM CHILDREN |
title_sort |
evaluation of a rapid screening assay for bacterial identification (dot-elisa) in fecal samples from children |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1997 |
url |
https://doi.org/10.1590/S0036-46651997000100005 https://doaj.org/article/7b4c239cb72046678f85a549e0f09362 |
long_lat |
ENVELOPE(-26.750,-26.750,-76.150,-76.150) |
geographic |
Aguda Arctic |
geographic_facet |
Aguda Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 39, Iss 1, p 21 (1997) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651997000100005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651997000100005 0036-4665 1678-9946 https://doaj.org/article/7b4c239cb72046678f85a549e0f09362 |
op_doi |
https://doi.org/10.1590/S0036-46651997000100005 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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39 |
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21 |
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