EVALUATION OF A RAPID SCREENING ASSAY FOR BACTERIAL IDENTIFICATION (DOT-ELISA) IN FECAL SAMPLES FROM CHILDREN

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Etelvina BOCCATTO, Sânia Alves dos SANTOS, Neusa Augusta Oliveira MAZIERI, Dahir Ramos de ANDRADE, Francisco Roque CARRAZZA, Roberto Mitio YANAGUITA
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 1997
• Subjects: Infantile diarrhea; Enteropathogens; Fecal antigens; Stool culture; Dot-ELISA; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Aguda; Arctic
• Online Access: https://doi.org/10.1590/S0036-46651997000100005; https://doaj.org/article/7b4c239cb72046678f85a549e0f09362

With the objective of standardizing a Dot Enzyme-Linked Immunosorbent Assay (Dot-ELISA) to detect antigens of fecal bacterial enteropathogens, 250 children, aged under 36 months and of both sexes, were studied; of which 162 had acute gastroenteritis. The efficacy of a rapid screening assay for bacterial enteropathogens (enteropathogenic Escherichia coli "EPEC", enteroinvasive Escherichia coli "EIEC", Salmonella spp. and Shigella spp.) was evaluated. The fecal samples were also submitted to a traditional method of stool culture for comparison. The concordance index between the two techniques, calculated using the Kappa (k) index for the above mentioned bacterial strains was 0.8859, 0.9055, 0.7932 and 0.7829 respectively. These values express an almost perfect degree of concordance for the first two and substantial concordance for the latter two, thus enabling this technique to be applied in the early diagnosis of diarrhea in infants. With a view to increasing the sensitivity and specificity of this immunological test, a study was made of the antigenic preparations obtained from two types of treatment: 1) deproteinization by heating; 2) precipitation and concentration of the lipopolysaccharide antigen (LPS) using an ethanol-acetone solution, which was then heated in the presence of sodium EDTA Com o objetivo de padronizar um Dot Enzyme-Linked Immunosorbent Assay (Dot-ELISA) para a detecção de antígenos de enteropatógenos bacterianos fecais, estudaram-se 250 crianças, abaixo de 36 meses de idade, de ambos os sexos, 162 portadoras de gastroenterite aguda. Avaliou-se a eficácia de um teste rápido para bactérias enteropatógenas (Escherichia coli enteropatogênica "EPEC", Escherichia coli enteroinvasora" EIEC", Salmonella spp. e Shigella spp.). As amostras fecais foram também submetidas à metodologia tradicional de coprocultura para comparação. Os índices de concordância entre as 2 técnicas, calculado através do índice Kappa (k) para as cepas bacterianas foram 0,8859; 0,9055; 0,7932 e 0,7829 respectivamente. Estes ...