Histochemical studies of the common bile duct in reindeer
Histochemical characteristics of bile duct mucosubstances, mast cells and globule leucocytes have not previously been described in the reindeer. Therefore various staining methods were applied on 1 to 6 specimens cut from formaline or Bouin-fixed histological blocks of the ductus hepaticus communis...
| Published in: | Rangifer |
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| Main Authors: | , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Septentrio Academic Publishing
1990
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| Subjects: | |
| Online Access: | https://doi.org/10.7557/2.10.1.785 https://doaj.org/article/7af3b59f1fc04277abc7c6904ab206d0 |
| Summary: | Histochemical characteristics of bile duct mucosubstances, mast cells and globule leucocytes have not previously been described in the reindeer. Therefore various staining methods were applied on 1 to 6 specimens cut from formaline or Bouin-fixed histological blocks of the ductus hepaticus communis from 20 reindeer. The present study showed that bile duct mucins include neutral, carboxy- and sulphomucins located chiefly in goblet cells and in the deep glands and as a thin superficial layer covering the surface epithelium. PAS-reactivity was diastase resistant, indicating that glycogen was not demonstrable in the epithelial layer of reindeer, contrary to previous studies e.g., on carnivores. Furthermore, carboxymucins were sialidase-resistant, as sialic acid could not be identified in the present material. Certain differences were noted in the appearance and composition of intracytoplasmic granules and globules of mast cells and globule leucocytes, respectively. The mucosubstances of the mast cell contained sulphate groups indicative of sulphomucins while both neutral, carboxy- and sulphomucins were identifiable in globule leucocytes. However, due to the sensitivity of mast cells and globule leucocytes to postmortal changes the above interpretations need to be confirmed by further studies. Histokemiallinen tutkimus yhteisen sappikaytavan rakenteesta porolla. Abstract in Finnish / Yhteenveto: Yhteisen sappikaytavan (ductus hepaticus communis) seinaman epiteelisolukon ja syotto- ja kerassolujen lima-aineiden koostumusta porolla ei ole aikaisemmin kuvattu. Sen vuoksi katsottiin aiheelliseksi suorit-taa histokemiallinen tutkimus formaliini- ja Bouin-kovetetusta histologisesta aineistosta, jonka kirjoittajat ovat julkaisseet aikaisemmin. Aineisto on peraisin 20 porosta. Erilaisia histokemiallisia varjayksia sovellettiin 1-6:een valikoituun leikkeeseen tarkoituksella analysoida nimenomaan lima-aineiden koostumusta. Tutkimuksissa ilmeni, etta sappikaytavien erittama lima koostuu neutraaleista, karboksy- ja ... |
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