Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy

This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax d...

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Bibliographic Details
Published in:Molecules
Main Authors: Elena Shumilina, Alessandra Ciampa, Trine Ytrestøyl, Alexander Dikiy
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2020
Subjects:
carotenoid
astaxanthin
Atlantic salmon
fish diet
fish feed
plant proteins
Organic chemistry
QD241-441
Online Access:https://doi.org/10.3390/molecules25081769
https://doaj.org/article/79f27b8ea9be4a1eb82b835a1edb188b
Description
Summary:This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax detection and quantification. The use of the 13 C-enriched Ax allowed the assessment of short-time Ax metabolism. The substitution of fish oil and meal in fish feed on plant analogs and the addition of MPL caused further catabolism and decrease of Ax accumulation in hepatocytes from 17 to about 6 mg/kg or to almost zero in the case of DHA addition. Signals assignment of the native and 13 C-enriched astaxanthin in acetone were performed using 1D and 2D NMR spectra.

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