Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection

Abstract Relative quantification using RT-qPCR is a widely used method for transcription profiling. Transcript levels of target genes in fish after experimental infection is often reported without documentation of stably transcribed reference genes. We present results demonstrating that transcriptio...

Full description

Bibliographic Details
Published in:Veterinary Research
Main Authors: Løvoll Marie, Austbø Lars, Jørgensen Jorunn B, Rimstad Espen, Frost Petter
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2011
Subjects:
Veterinary medicine
SF600-1100
Atlantic salmon
Online Access:https://doi.org/10.1186/1297-9716-42-8
https://doaj.org/article/78d326449d25490b8c7be4faf1a7f15e
Description
Summary:Abstract Relative quantification using RT-qPCR is a widely used method for transcription profiling. Transcript levels of target genes in fish after experimental infection is often reported without documentation of stably transcribed reference genes. We present results demonstrating that transcription of typically used reference genes in Atlantic salmon is not stable during experimental infection with salmon pancreas disease virus (SPDV). Transcript levels 0 to 6 weeks after challenge revealed statistically significant changes between time-points that corresponded with a peak in viral load 3 weeks after challenge. The results emphasize the need for thorough method validation prior to transcriptional studies during viral infections.

Similar Items